Table 2. Identification of antibodies in... | American Society of Hematology

Table 2.

Identification of antibodies in sera from individuals 1 to 12

	Origin of immunization	Serum reactivity							Titer			Antibodies in serum
	Origin of immunization	Common RBCs	Rh_null RBCs	D- RBCs	RH:−18 RBCs	ceMO RBCs	Other RBCs	After absorption on DccEE RBCs	ddccee RBCs	DCCee RBCs	DccEE RBCs	Antibodies in serum
Group A
1	2 pregnancies transfusion	+	0	0	nt	+	9 +	anti-e–like	256	128	256	anti-RH18, anti-E
2	2 pregnancies	+	0	+	nt	nt		anti-e–like	256	64	64	anti-RH18, anti-D
3	3 pregnancies	+	0	+	0	nt	5 0	anti-e–like	16	32	64	anti-RH18, anti-D
4	3 pregnancies	+	0	+	0	nt		anti-e–like	8	4	4	anti-RH18, anti-D
5	3 pregnancies	+	0	0	0	+	3 +	anti-e–like, anti-C	32	256	4	anti-RH18, anti-C
6	2 pregnancies	+	0	0	nt	+		anti-e–like	64	16	64	anti-RH18, anti-E
7	1 pregnancy transfusion	+	0	0	0	+		anti-e–like, anti-C	256	256	256	anti-RH18, anti-C, anti-Fy^a, anti-S
8	transfusion	+	0	0	0	nt		anti-e–like	2000	512	512	anti-RH18
Group B
9	2 pregnancies transfusion	+	0	+	+	nt		anti-Ce–like	32	8000	8000	anti-RH34, anti-D
10	transfusion	+	0	0	+	+		anti-Ce–like	64	128	16	anti-RH34
Group C
11	2 pregnancies	0/+	0	0	nt	0	9 +	nt	2	1	0	anti-e, ce
12	4 pregnancies	0/+	0	0	+	+	9 +	nt	16	64	0	anti-e, Ce

	Origin of immunization	Serum reactivity							Titer			Antibodies in serum
	Origin of immunization	Common RBCs	Rh_null RBCs	D- RBCs	RH:−18 RBCs	ceMO RBCs	Other RBCs	After absorption on DccEE RBCs	ddccee RBCs	DCCee RBCs	DccEE RBCs	Antibodies in serum
Group A
1	2 pregnancies transfusion	+	0	0	nt	+	9 +	anti-e–like	256	128	256	anti-RH18, anti-E
2	2 pregnancies	+	0	+	nt	nt		anti-e–like	256	64	64	anti-RH18, anti-D
3	3 pregnancies	+	0	+	0	nt	5 0	anti-e–like	16	32	64	anti-RH18, anti-D
4	3 pregnancies	+	0	+	0	nt		anti-e–like	8	4	4	anti-RH18, anti-D
5	3 pregnancies	+	0	0	0	+	3 +	anti-e–like, anti-C	32	256	4	anti-RH18, anti-C
6	2 pregnancies	+	0	0	nt	+		anti-e–like	64	16	64	anti-RH18, anti-E
7	1 pregnancy transfusion	+	0	0	0	+		anti-e–like, anti-C	256	256	256	anti-RH18, anti-C, anti-Fy^a, anti-S
8	transfusion	+	0	0	0	nt		anti-e–like	2000	512	512	anti-RH18
Group B
9	2 pregnancies transfusion	+	0	+	+	nt		anti-Ce–like	32	8000	8000	anti-RH34, anti-D
10	transfusion	+	0	0	+	+		anti-Ce–like	64	128	16	anti-RH34
Group C
11	2 pregnancies	0/+	0	0	nt	0	9 +	nt	2	1	0	anti-e, ce
12	4 pregnancies	0/+	0	0	+	+	9 +	nt	16	64	0	anti-e, Ce

In this table, samples of individuals 1–12 are divided into 3 groups (A, B, C) according to similar results of Rh antibody characterization. Group D individuals (13-17) were not immunized. Immunization challenge is indicated for each individual. Serum reactivity was studied on common RBCs. When sera did not react with all common RBCs (sera 11-12: 0/+), adsorption studies were not performed. For the other sera, after adsorption on DccEE RBCs, the specificity found is followed by “like” when the individual expressed the specificity of the antibody. All sera were tested on Rh_null, D- - and titration performed on ddccee, DCCee, and DccEE RBCs. RH:−18 from the SCARF were tested with some sera. ceMO RBCs (typed Dccee) were not tested when an anti-D was present in the serum. Some sera also were tested with RBCs from these study (“Other RBCs”): serum 3 was tested with RBCs 5; serum 5 with RBCs 3; sera 1, 11, and 12 with RBCs 9 (defined as RH:−34 in this study). The last column indicates all the antibodies found in each serum.

nt indicates not tested.

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