Table 1. Clonal analysis of B- and... | American Society of Hematology

Table 1.

Clonal analysis of B- and NK-differentiation potential

Experiment	Sorted cells^*	B-only clones^†	NK-only clones^‡	B+NK clones^1-153
1	Lin⁻c-kit^LoFlk-2⁺	59/374 (1:6.3)	15/374 (1:24.9)	4/374 (1:93.9)
2	Lin⁻ c-kit^LoFlk-2⁺	18/192 (1:10.7)	10/192 (1:19.2)	2/192 (1:96.0)
3	Lin⁻ c-kit^Lo Flk-2⁺IL-7Rα⁺	104/384 (1:3.7)	13/384 (1:29.5)	6/384 (1:64)

Experiment	Sorted cells^*	B-only clones^†	NK-only clones^‡	B+NK clones^1-153
1	Lin⁻c-kit^LoFlk-2⁺	59/374 (1:6.3)	15/374 (1:24.9)	4/374 (1:93.9)
2	Lin⁻ c-kit^LoFlk-2⁺	18/192 (1:10.7)	10/192 (1:19.2)	2/192 (1:96.0)
3	Lin⁻ c-kit^Lo Flk-2⁺IL-7Rα⁺	104/384 (1:3.7)	13/384 (1:29.5)	6/384 (1:64)

Each cell of the table shows the number of clones of a given type that were detected/total number of seeded wells, followed by the calculated frequency.

Single cells with the indicated characteristics were sorted and cultured as described in “Materials and methods.” Individual wells were harvested either 11 days (experiments 1 and 2) or 11 and 15 days later (experiment 3) and characterized by flow cytometry.

†

Cultures containing CD19⁺ cells but less than 5% DX5⁺ cells were counted as B-only clones.

‡

Cultures containing DX5⁺ cells but less than 5% CD19⁺ cells were scored as NK-only clones.

F1-153

Cultures containing at least 10% of both CD19⁺ and DX5⁺ cells were counted as B+NK clones.

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