Table 2. Divergence of B- and NK-cell... | American Society of Hematology

Table 2.

Divergence of B- and NK-cell lineages in 2-step cultures

Sorted cells^*	CD122⁻CD45R⁻		CD122⁻CD45R⁺		CD122⁺CD45R⁻		CD122⁺ CD45R⁺
Yield in the 1st culture^†	0.92		1.66		0.21		0.32
After 2nd culture^‡	B	NK	B	NK	B	NK	B	NK
Yield/input (2nd culture)^2-153	1.22	0.85	6.04	1.00	0.41	19.91	0.29	17.58
Yield/1 Lin⁻ c-kit^Locell^2-154	1.13	0.79	10.03	1.67	0.09	4.15	0.09	5.58

Sorted cells^*	CD122⁻CD45R⁻		CD122⁻CD45R⁺		CD122⁺CD45R⁻		CD122⁺ CD45R⁺
Yield in the 1st culture^†	0.92		1.66		0.21		0.32
After 2nd culture^‡	B	NK	B	NK	B	NK	B	NK
Yield/input (2nd culture)^2-153	1.22	0.85	6.04	1.00	0.41	19.91	0.29	17.58
Yield/1 Lin⁻ c-kit^Locell^2-154	1.13	0.79	10.03	1.67	0.09	4.15	0.09	5.58

Lin⁻ c-kit^Lo bone marrow cells were cultured with IL-7, FL, and SCF for 3 days and sorted by surface markers as shown.

†

These values represent numbers of cells of the indicated types harvested from primary cultures divided by the input cell numbers.

‡

Each subset of cells that was harvested from the primary cultures was recultured with IL-7, FL, SCF, and IL-15 for 5 days. B-lineage (CD19⁺) and NK-lineage (DX5⁺) cells were then determined by flow cytometry.

F2-153

The yields of B- and NK-lineage cells in secondary cultures were determined by dividing harvested cells by the input cell numbers.

F2-154

These values provide an index of how effective each type of progenitor was in producing lymphocytes. They were calculated by multiplying final cell numbers harvested from the secondary culture by numbers of cells used to start the primary cultures.

View Large

This Feature Is Available To Subscribers Only