Table 1. Immunohistochemical analysis on... | American Society of Hematology

Table 1.

Immunohistochemical analysis on plastic and paraffin-embedded specimen

Antigen	Source	Plastic sections		Paraffin sections
Antigen	Source	Pretreatment	Dilution	Pretreatment	Dilution
Myeloperoxidase	DAKO^†	Protease	1:5	Protease	1:10
CD68 (KP1)	DAKO^†	Protease	1:60	Protease	1:100
CD45 (LC)	DAKO^†	None	1:20	None	1:100
CD3	DAKO^†	Microwave	1:50	Protease	1:300
CD20 (L26)	Immunotech^‡	Microwave	1:2	Microwave	1:20
CD79a (MB1)	Laboserv^1-153	Microwave	1:10	Microwave	1:20
Plasma cells	DAKO^†	Microwave	1:10	Microwave	1:30
κ light chain^*	DAKO^†	Protease	1:10 000	Protease	1:15 000
λ light chain^*	DAKO^†	Protease	1:10 000	Protease	1:15 000
IgA^*	DAKO^†	Protease	1:7 000	Protease	1:6 000
IgD^*	DAKO^†	—	—	Microwave	1:100
IgG^*	DAKO^†	Protease	1:2 000	Protease	1:7 000
IgM^*	DAKO^†	—	—	Protease	1:3 000

Antigen	Source	Plastic sections		Paraffin sections
Antigen	Source	Pretreatment	Dilution	Pretreatment	Dilution
Myeloperoxidase	DAKO^†	Protease	1:5	Protease	1:10
CD68 (KP1)	DAKO^†	Protease	1:60	Protease	1:100
CD45 (LC)	DAKO^†	None	1:20	None	1:100
CD3	DAKO^†	Microwave	1:50	Protease	1:300
CD20 (L26)	Immunotech^‡	Microwave	1:2	Microwave	1:20
CD79a (MB1)	Laboserv^1-153	Microwave	1:10	Microwave	1:20
Plasma cells	DAKO^†	Microwave	1:10	Microwave	1:30
κ light chain^*	DAKO^†	Protease	1:10 000	Protease	1:15 000
λ light chain^*	DAKO^†	Protease	1:10 000	Protease	1:15 000
IgA^*	DAKO^†	Protease	1:7 000	Protease	1:6 000
IgD^*	DAKO^†	—	—	Microwave	1:100
IgG^*	DAKO^†	Protease	1:2 000	Protease	1:7 000
IgM^*	DAKO^†	—	—	Protease	1:3 000

Polyspecific primary antibodies.

DAKO Diagnostika, Hamburg, Germany.

Coulter-Immunotech Diagnostics, Hamburg, Germany.

Laboserve, Gießen, Germany.