PMN transendothelial migration time and junction protein open time
. | VE-cadherin . | PECAM-1 . | ||
---|---|---|---|---|
. | Tt . | To . | Tt . | To . |
Culture | ||||
Bicellular boundary | 48.2 ± 3.3 (22) | 50.9 ± 3.1 (22) | 44.7 ± 2.6 (19) | 46.3 ± 2.9 (19) |
Multicellular corner | 49.0 ± 1.7 (79) | 52.9 ± 1.7 (79) | 47.4 ± 1.9 (54) | 48.9 ± 1.9 (54) |
Tissue | ||||
Bicellular boundary | 79 ± 9 (16) | 86 ± 9 (16) | 79 ± 11 (13) | 88 ± 11 (13) |
Multicellular corner | 81 ± 5 (58) | 86 ± 5 (58) | 79 ± 5 (45) | 87 ± 5 (45) |
. | VE-cadherin . | PECAM-1 . | ||
---|---|---|---|---|
. | Tt . | To . | Tt . | To . |
Culture | ||||
Bicellular boundary | 48.2 ± 3.3 (22) | 50.9 ± 3.1 (22) | 44.7 ± 2.6 (19) | 46.3 ± 2.9 (19) |
Multicellular corner | 49.0 ± 1.7 (79) | 52.9 ± 1.7 (79) | 47.4 ± 1.9 (54) | 48.9 ± 1.9 (54) |
Tissue | ||||
Bicellular boundary | 79 ± 9 (16) | 86 ± 9 (16) | 79 ± 11 (13) | 88 ± 11 (13) |
Multicellular corner | 81 ± 5 (58) | 86 ± 5 (58) | 79 ± 5 (45) | 87 ± 5 (45) |
Numbers in parentheses represent the number of transmigration events counted. Tt, transmigration time (seconds) determined by tracing the morphologic changes of PMNs; To, junction protein open time (seconds) determined by tracing the movements of either junction protein