Partial inhibition of colony formation by BCR-ABL-transduced +/+ CFCs and LTC-IC-derived CFCs in the presence of antibodies to IL-3, GM-CSF, and G-CSF
| Antibodies added to CFC assays . | Origin of CFCs . | No. of colonies* . | % inhibition† . |
|---|---|---|---|
| Control‡ | Sca-1+lin−GFP+cells | 150 ± 30 | — |
| Anti–IL-3 | 97 ± 22 | 35 ± 5 | |
| Anti–GM-CSF | 114 ± 26 | 25 ± 4 | |
| Anti–G-CSF | 120 ± 26 | 20 ± 3 | |
| Anti–IL-3 + anti–GM-CSF | 81 ± 14 | 46 ± 3 | |
| Anti–IL-3 + anti–GM-CSF + anti–G-CSF | 80 ± 20 | 48 ± 4 | |
| Control‡ | LTC-IC | 2000 ± 200 | — |
| Anti–IL-3 | 1290 ± 200 | 36 ± 4 | |
| Anti–GM-CSF | 1350 ± 180 | 31 ± 3 | |
| Anti–G-CSF | 1688 ± 142 | 16 ± 2 | |
| Anti–IL-3 + anti–GM-CSF | 1000 ± 50 | 49 ± 3 | |
| Anti–IL-3 + anti–GM-CSF + anti–G-CSF | 994 ± 90 | 50 ± 4 |
| Antibodies added to CFC assays . | Origin of CFCs . | No. of colonies* . | % inhibition† . |
|---|---|---|---|
| Control‡ | Sca-1+lin−GFP+cells | 150 ± 30 | — |
| Anti–IL-3 | 97 ± 22 | 35 ± 5 | |
| Anti–GM-CSF | 114 ± 26 | 25 ± 4 | |
| Anti–G-CSF | 120 ± 26 | 20 ± 3 | |
| Anti–IL-3 + anti–GM-CSF | 81 ± 14 | 46 ± 3 | |
| Anti–IL-3 + anti–GM-CSF + anti–G-CSF | 80 ± 20 | 48 ± 4 | |
| Control‡ | LTC-IC | 2000 ± 200 | — |
| Anti–IL-3 | 1290 ± 200 | 36 ± 4 | |
| Anti–GM-CSF | 1350 ± 180 | 31 ± 3 | |
| Anti–G-CSF | 1688 ± 142 | 16 ± 2 | |
| Anti–IL-3 + anti–GM-CSF | 1000 ± 50 | 49 ± 3 | |
| Anti–IL-3 + anti–GM-CSF + anti–G-CSF | 994 ± 90 | 50 ± 4 |
From assays of 103 Sca-1+lin− GFP+ cells assayed directly or after their maintenance in LTCs for 4 weeks. CFC assays were performed in FCS-containing methylcellulose medium without added growth factors. Antibodies were added as shown at 10 μg/mL.
Calculated as a percent of the value obtained in the control assays in the same experiment.
Control antibodies were sheep or rabbit IgG as appropriate.
Data shown are the mean ± SEM of values obtained from 3 independent experiments.
CFC indicates colony-forming cell; LTC-IC, long-term culture-initiating cell; IL-3, interleukin 3; GM-CSF, granulocyte macrophage–colony-stimulating factor; G-CSF, granulocyte–colony-stimulating factor; FCS, fetal calf serum.