Effect of a microbial stimulus and antigen-mediated T-cell stimulation on splenic DC lifespan
| Stimulus . | % BrdU-positive cells, day 2 . | |||
|---|---|---|---|---|
| Total DC . | CD4−CD8−DC . | CD4+CD8−DC . | CD4−CD8+DC . | |
| Uninjected | 23 ± 5 | 18 ± 4 | 12 ± 4 | 39 ± 5 |
| OVA | 36 ± 10 | 34 ± 9 | 24 ± 4 | 68 ± 6 |
| OVA and LPS | 48 ± 8 | 35 ± 8 | 50 ± 1 | 69 ± 4 |
| BSA | 27 ± 6 | 29 ± 13 | 21 ± 8 | 39 ± 6 |
| BSA and LPS | 55 ± 4 | 46 ± 5 | 50 ± 1 | 69 ± 1 |
| Stimulus . | % BrdU-positive cells, day 2 . | |||
|---|---|---|---|---|
| Total DC . | CD4−CD8−DC . | CD4+CD8−DC . | CD4−CD8+DC . | |
| Uninjected | 23 ± 5 | 18 ± 4 | 12 ± 4 | 39 ± 5 |
| OVA | 36 ± 10 | 34 ± 9 | 24 ± 4 | 68 ± 6 |
| OVA and LPS | 48 ± 8 | 35 ± 8 | 50 ± 1 | 69 ± 4 |
| BSA | 27 ± 6 | 29 ± 13 | 21 ± 8 | 39 ± 6 |
| BSA and LPS | 55 ± 4 | 46 ± 5 | 50 ± 1 | 69 ± 1 |
OVA-specific, TCR-transgenic TO-II mice were injected intravenously with 50 μg OVA or BSA as a control, with or without 5 μg bacterial LPS. Concurrently, the mice were given 1 mg BrdU intraperitoneally followed by continuous administration of BrdU-treated water for 2 days. DCs were then isolated and BrdU labeling was determined.