Colony-forming activities of mobilized PBSCs
Mice . | No. . | CFU-Cs, /105 cells . | CFU-Ss, d 12, /105 cells . |
---|---|---|---|
BXSB | 4 | 161.0 ± 5.3* | 38.7 ± 7.4* |
BALB/c | 3 | 128.0 ± 6.0 | 32.3 ± 9.3 |
B6 | 3 | 102.2 ± 2.0 | 27.0 ± 6.6 |
Mice . | No. . | CFU-Cs, /105 cells . | CFU-Ss, d 12, /105 cells . |
---|---|---|---|
BXSB | 4 | 161.0 ± 5.3* | 38.7 ± 7.4* |
BALB/c | 3 | 128.0 ± 6.0 | 32.3 ± 9.3 |
B6 | 3 | 102.2 ± 2.0 | 27.0 ± 6.6 |
CFU assays were carried out when mice were 8 weeks old. For the CFU-C assay, 2 × 104 cells were cultured in 1 mL semisolid medium containing 100 U/mL mIL-3 and 2 U/mL Epo for 7 days. For the CFU-S assay, 5 × 104 cells were injected into irradiated syngeneic mice. Data are shown as the mean ± SD.
CFU-C indicates culture CFUs; and CFU-S, spleen CFUs.
P < .05 compared with other groups.