Clinicohematologic characteristics and FLT3/ITD mutation patterns in AML patients carrying more than one mutation at diagnosis or first relapse
| Patient . | Age/sex . | FAB subtype . | Chromosome/ genetic abnormality . | % leukemic blasts in BM . | Length of FLT3/ITD (bp) . | Location of duplication . | FLT3/ITD level (%) . | % FLT3/ITD expression by RT-PCR . | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Diagnosis . | Relapse . | Diagnosis . | Relapse . | Diagnosis . | Relapse . | ||||||
| 18 | 69/M | M1 | 46,XY | 90.2 | 90.0 | 54 | 1774-1827 (ex 14) | 0 | 6.1 | 0 | 0 |
| 141 | 1792-1842 (ex 14 and 15)+1-90(int 14) | 0 | 28.7 | 0 | 48.3 | ||||||
| 19 | 26/F | M2 | 46,XX | 43.6 | 50.4 | 36 | 1789-1824 (ex 14) | 0.7 | 26.6 | 2.0 | 41.5 |
| 57 | 1748-1804 (ex 14) | 3.3 | 0 | 11.0 | 0 | ||||||
| 20 | 22/F | M2 | 46,XX | 68.3 | 87.8 | 36 | 1792-1827 (ex 14) | 1.0 | 0 | 0 | 0 |
| 69 | 1715-1783 (ex 14) | 27.0 | 45.5 | 31.9 | 41.3 | ||||||
| 21 | 49/M | M2 | 46,XY,t(16;16) (p13;q22) | 94.0 | 72.8 | 93 | 1715-1801 (ex 14)+ins 6 bp | 2.8 | 0 | 0 | 0 |
| 168 | 1765-1837 (ex 14)+1-90(int 14) +ins 5 bp | 7.4 | 23.7 | 21.2 | 40.1 | ||||||
| 22 | 64/M | M2 | ND | 66.1 | 94.3 | 42 | 1770-1811 (ex 14) | 7.2 | 0 | 10.1 | 0 |
| 54 | 1742-1795 (ex 14) | 34.0 | 47.5 | 25.0 | 52.7 | ||||||
| 23 | 43/M | M4 | 46,XY | 55.7 | 90.0 | 36 | 1782-1817 (ex 14) | 27.6 | 4.7 | 33.5 | 5.0 |
| 72 | 1759-1830 (ex 14) | 4.2 | 0 | 17.5 | 0 | ||||||
| 102 | 1733-1829 (ex 14)+ins 5 bp | 0 | 42.5 | 0 | 42.4 | ||||||
| 24 | 47/F | M2 | ND | 55.8 | 54.8 | 21 | 1780-1800 (ex 14) | 1.5 | 0 | 2.8 | 0 |
| 39 | 1770-1808 (ex 14) | 9.6 | 0 | 12.6 | 0 | ||||||
| 63 | 1751-1813 (ex 14) | 2.2 | 53.7 | 2.3 | 90.8 | ||||||
| 25 | 22/F | M0 | No metaphase | 99.0 | 85.6 | 63 | 1725-1782 (ex 14)+ ins 5 bp | 7.5 | 7.4 | 3.4 | 0 |
| 240 | 1739-1885 (ex 14 and 15)+1-90(int 14) +ins 3 bp | 0 | 17.3 | 0 | 10.2 | ||||||
| Patient . | Age/sex . | FAB subtype . | Chromosome/ genetic abnormality . | % leukemic blasts in BM . | Length of FLT3/ITD (bp) . | Location of duplication . | FLT3/ITD level (%) . | % FLT3/ITD expression by RT-PCR . | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Diagnosis . | Relapse . | Diagnosis . | Relapse . | Diagnosis . | Relapse . | ||||||
| 18 | 69/M | M1 | 46,XY | 90.2 | 90.0 | 54 | 1774-1827 (ex 14) | 0 | 6.1 | 0 | 0 |
| 141 | 1792-1842 (ex 14 and 15)+1-90(int 14) | 0 | 28.7 | 0 | 48.3 | ||||||
| 19 | 26/F | M2 | 46,XX | 43.6 | 50.4 | 36 | 1789-1824 (ex 14) | 0.7 | 26.6 | 2.0 | 41.5 |
| 57 | 1748-1804 (ex 14) | 3.3 | 0 | 11.0 | 0 | ||||||
| 20 | 22/F | M2 | 46,XX | 68.3 | 87.8 | 36 | 1792-1827 (ex 14) | 1.0 | 0 | 0 | 0 |
| 69 | 1715-1783 (ex 14) | 27.0 | 45.5 | 31.9 | 41.3 | ||||||
| 21 | 49/M | M2 | 46,XY,t(16;16) (p13;q22) | 94.0 | 72.8 | 93 | 1715-1801 (ex 14)+ins 6 bp | 2.8 | 0 | 0 | 0 |
| 168 | 1765-1837 (ex 14)+1-90(int 14) +ins 5 bp | 7.4 | 23.7 | 21.2 | 40.1 | ||||||
| 22 | 64/M | M2 | ND | 66.1 | 94.3 | 42 | 1770-1811 (ex 14) | 7.2 | 0 | 10.1 | 0 |
| 54 | 1742-1795 (ex 14) | 34.0 | 47.5 | 25.0 | 52.7 | ||||||
| 23 | 43/M | M4 | 46,XY | 55.7 | 90.0 | 36 | 1782-1817 (ex 14) | 27.6 | 4.7 | 33.5 | 5.0 |
| 72 | 1759-1830 (ex 14) | 4.2 | 0 | 17.5 | 0 | ||||||
| 102 | 1733-1829 (ex 14)+ins 5 bp | 0 | 42.5 | 0 | 42.4 | ||||||
| 24 | 47/F | M2 | ND | 55.8 | 54.8 | 21 | 1780-1800 (ex 14) | 1.5 | 0 | 2.8 | 0 |
| 39 | 1770-1808 (ex 14) | 9.6 | 0 | 12.6 | 0 | ||||||
| 63 | 1751-1813 (ex 14) | 2.2 | 53.7 | 2.3 | 90.8 | ||||||
| 25 | 22/F | M0 | No metaphase | 99.0 | 85.6 | 63 | 1725-1782 (ex 14)+ ins 5 bp | 7.5 | 7.4 | 3.4 | 0 |
| 240 | 1739-1885 (ex 14 and 15)+1-90(int 14) +ins 3 bp | 0 | 17.3 | 0 | 10.2 | ||||||
ND indicates not done.