Oligonucleotide primer used in PCR analysis
| Oligonucleotide primer . | Primer . | Sequence . |
|---|---|---|
| FLT3/ITD | F | 5′-GCAATTTAGGTATGAAAGCCAGC-3′ |
| FLT3/ITD | R | 5′-CTTTCAGCATTTTGACGGCAACC-3′ |
| FLT3/D835 | F | 5′-CCGCCAGGAACGTGCTTG-3′ |
| FLT3/D835 | R | 5′-GCAGCCTCACATTGCCCC-3′ |
| N-RAS exon 1 (DNA) | F | 5′-GACTGAGTACAAACTGGTGG-3′ |
| R | 5′-TGCATAACTGAATGTATACCC-3′ | |
| N-RAS exon 2 (DNA) | F | 5′-CAAGTGGTTATAGATGGTGAAACC-3′ |
| R | 5′-AAGATCATCCTTTCAGAGAAAATAAT-3′ | |
| N-RAS exons 1 + 2 (cDNA) | F | 5′-CTGTCCAAAGCAGAGGCAGTG-3′ |
| R | 5′-AGGCTTCCTCTGTGTATTTGCC-3′ | |
| KB9 | F | 5′-TGCAAAGGCTTGGAGGGCTGATG-3′ |
| R | 5′-ATCTCGGACAACAGCAGGCCTCG-3′ | |
| D21S270 | F | 5′-GAAATGTTTTAATAAATGGTGGTTA-3′ |
| R | 5′-ACAAAGTTATGGTCAAGGGG-3′ | |
| D21S65 | F | 5′-CCGAAAACTTACTGGAGAAC-3′ |
| R | 5′-GATCATCCAGGAATCACCAA-3′ |
| Oligonucleotide primer . | Primer . | Sequence . |
|---|---|---|
| FLT3/ITD | F | 5′-GCAATTTAGGTATGAAAGCCAGC-3′ |
| FLT3/ITD | R | 5′-CTTTCAGCATTTTGACGGCAACC-3′ |
| FLT3/D835 | F | 5′-CCGCCAGGAACGTGCTTG-3′ |
| FLT3/D835 | R | 5′-GCAGCCTCACATTGCCCC-3′ |
| N-RAS exon 1 (DNA) | F | 5′-GACTGAGTACAAACTGGTGG-3′ |
| R | 5′-TGCATAACTGAATGTATACCC-3′ | |
| N-RAS exon 2 (DNA) | F | 5′-CAAGTGGTTATAGATGGTGAAACC-3′ |
| R | 5′-AAGATCATCCTTTCAGAGAAAATAAT-3′ | |
| N-RAS exons 1 + 2 (cDNA) | F | 5′-CTGTCCAAAGCAGAGGCAGTG-3′ |
| R | 5′-AGGCTTCCTCTGTGTATTTGCC-3′ | |
| KB9 | F | 5′-TGCAAAGGCTTGGAGGGCTGATG-3′ |
| R | 5′-ATCTCGGACAACAGCAGGCCTCG-3′ | |
| D21S270 | F | 5′-GAAATGTTTTAATAAATGGTGGTTA-3′ |
| R | 5′-ACAAAGTTATGGTCAAGGGG-3′ | |
| D21S65 | F | 5′-CCGAAAACTTACTGGAGAAC-3′ |
| R | 5′-GATCATCCAGGAATCACCAA-3′ |