Characterization of bone marrow chimeras
| Group; no. of mice (graft→host) . | % Total thymocytes (% Ly5. within the subset) . | % Total splenocytes (% Ly5. within the subset) . | ||||
|---|---|---|---|---|---|---|
| CD4+CD8+ . | CD8+ . | CD4+ . | CD3−CD4− . | CD3+CD4− . | CD4+ . | |
| A; n = 2 | 79, 69 | 3.3, 2.8 | 17, 11 | 61, 60 | 11, 7 | 25, 25 |
| (5.2 wt→5.1 wt) | (<2) | (<2) | (<2) | (<2) | (21, 12) | (10, 18) |
| B; n = 4 | 81 ± 3 | 2.2 ± 0.5 | 11 ± 2 | 67 ± 3 | 6 ± 0.7 | 21 ± 1 |
| (5.2 pIV−/−→5.1 wt) | (<2) | (<2) | (<2) | (< 2) | (17 ± 2) | (12 ± 3) |
| C; n = 4 | 92 ± 2 | 2 ± 0.6 | 0.5 ± 0.3 | 83 ± 5 | 10 ± 3 | 2 ± 0.4 |
| (5.1 wt→5.2 pIV−/−) | (>98) | (>98) | (>98) | (>98) | (78 ± 6) | (65 ± 3) |
| D; n = 3 | 77 ± 2 | 2.6 ± 0.6 | 13 ± 0.3 | 65 ± 3 | 9 ± 0.1 | 21 ± 0.3 |
| (mix→5.1 wt) | (81 ± 4) | (77 ± 2) | (78 ± 2) | (43 ± 9) | (74 ± 10) | (72 ± 10) |
| E; n = 2 | 89, 88.2 | 2.9, 1.9 | 0.9, 0.6 | 83, 77 | 11.1, 10.5 | 2.5, 1.8 |
| (mix→5.2 pIV−/−) | (85, 87) | (84, 82) | (82, 81) | (53, 40) | (64, 55) | (53, 51) |
| F; n = 1 | 92 | 2.8 | 0.5 | 84 | 10 | 2.4 |
| (5.2 pIV−/−→5.2 pIV−/−) | (0) | (0) | (0) | (0) | (0) | (0) |
| Group; no. of mice (graft→host) . | % Total thymocytes (% Ly5. within the subset) . | % Total splenocytes (% Ly5. within the subset) . | ||||
|---|---|---|---|---|---|---|
| CD4+CD8+ . | CD8+ . | CD4+ . | CD3−CD4− . | CD3+CD4− . | CD4+ . | |
| A; n = 2 | 79, 69 | 3.3, 2.8 | 17, 11 | 61, 60 | 11, 7 | 25, 25 |
| (5.2 wt→5.1 wt) | (<2) | (<2) | (<2) | (<2) | (21, 12) | (10, 18) |
| B; n = 4 | 81 ± 3 | 2.2 ± 0.5 | 11 ± 2 | 67 ± 3 | 6 ± 0.7 | 21 ± 1 |
| (5.2 pIV−/−→5.1 wt) | (<2) | (<2) | (<2) | (< 2) | (17 ± 2) | (12 ± 3) |
| C; n = 4 | 92 ± 2 | 2 ± 0.6 | 0.5 ± 0.3 | 83 ± 5 | 10 ± 3 | 2 ± 0.4 |
| (5.1 wt→5.2 pIV−/−) | (>98) | (>98) | (>98) | (>98) | (78 ± 6) | (65 ± 3) |
| D; n = 3 | 77 ± 2 | 2.6 ± 0.6 | 13 ± 0.3 | 65 ± 3 | 9 ± 0.1 | 21 ± 0.3 |
| (mix→5.1 wt) | (81 ± 4) | (77 ± 2) | (78 ± 2) | (43 ± 9) | (74 ± 10) | (72 ± 10) |
| E; n = 2 | 89, 88.2 | 2.9, 1.9 | 0.9, 0.6 | 83, 77 | 11.1, 10.5 | 2.5, 1.8 |
| (mix→5.2 pIV−/−) | (85, 87) | (84, 82) | (82, 81) | (53, 40) | (64, 55) | (53, 51) |
| F; n = 1 | 92 | 2.8 | 0.5 | 84 | 10 | 2.4 |
| (5.2 pIV−/−→5.2 pIV−/−) | (0) | (0) | (0) | (0) | (0) | (0) |
FACS analysis of bone marrow chimeras obtained by grafting Ly 5.1 or Ly 5.2 wild-type (wt) donor cells or Ly 5.2 pIV−/− donor cells into hosts of the indicated phenotype.