Phenotypic characterization of cord-blood T cells
| . | % positive CD8 T cells . | % positive CD4 T cells . | ||
|---|---|---|---|---|
| Uninfected newborns . | Congenitally infected newborns . | Uninfected newborns . | Congenitally infected newborns . | |
| Surface activation markers | ||||
| CD45RO+ | 4.5 ± 1.1‡ (n = 24) | 33.9 ± 4.0† (n = 14) | 9.9 ± 1.6 | 13.6 ± 1.4* |
| HLA-DR+ | 1.2 ± 0.3 (n = 12) | 53.7 ± 6.3† (n = 13) | 0.8 ± 0.2 | 6.2 ± 1.2† |
| CD25+ | 0.34 ± 0.13 (n = 20) | 0.25 ± 0.11 (n = 4) | 11.4 ± 1.3 | 9.1 ± 3.0 |
| CD69+ | 5.5 ± 2.0 (n = 13) | 5.8 ± 1.8 (n = 6) | 4.5 ± 1.3 | 4.0 ± 1.1 |
| CD28− | 2.9 ± 0.8 (n = 5) | 26.1 ± 8.2* (n = 5) | 0.1 ± 0.0 | 1.6 ± 0.5* |
| Intracellular expression of effector/regulating molecules | ||||
| IFN-γ | 3.1 ± 1.7 (n = 10) | 22.5 ± 4.4† (n = 9) | 1.1 ± 0.6 | 2.6 ± 0.5* |
| TNF-α | 5.7 ± 2.5 (n = 5) | 20.0 ± 7.1* (n = 5) | 36.5 ± 9.0 | 37.3 ± 9.0 |
| IL-2 | 7.2 ± 2.9 (n = 4) | 8.6 ± 1.7 (n = 5) | 22.7 ± 7.7 | 23.7 ± 6.2 |
| IL-4 | 2.1 ± 0.8 (n = 5) | 2.5 ± 0.3 (n = 5) | 1.2 ± 0.5 | 2.0 ± 0.8 |
| Perforin | 4.0 ± 1.7 (n = 14) | 12.1 ± 4.2* (n = 11) | 0.4 ± 0.2 | 2.0 ± 0.5† |
| . | % positive CD8 T cells . | % positive CD4 T cells . | ||
|---|---|---|---|---|
| Uninfected newborns . | Congenitally infected newborns . | Uninfected newborns . | Congenitally infected newborns . | |
| Surface activation markers | ||||
| CD45RO+ | 4.5 ± 1.1‡ (n = 24) | 33.9 ± 4.0† (n = 14) | 9.9 ± 1.6 | 13.6 ± 1.4* |
| HLA-DR+ | 1.2 ± 0.3 (n = 12) | 53.7 ± 6.3† (n = 13) | 0.8 ± 0.2 | 6.2 ± 1.2† |
| CD25+ | 0.34 ± 0.13 (n = 20) | 0.25 ± 0.11 (n = 4) | 11.4 ± 1.3 | 9.1 ± 3.0 |
| CD69+ | 5.5 ± 2.0 (n = 13) | 5.8 ± 1.8 (n = 6) | 4.5 ± 1.3 | 4.0 ± 1.1 |
| CD28− | 2.9 ± 0.8 (n = 5) | 26.1 ± 8.2* (n = 5) | 0.1 ± 0.0 | 1.6 ± 0.5* |
| Intracellular expression of effector/regulating molecules | ||||
| IFN-γ | 3.1 ± 1.7 (n = 10) | 22.5 ± 4.4† (n = 9) | 1.1 ± 0.6 | 2.6 ± 0.5* |
| TNF-α | 5.7 ± 2.5 (n = 5) | 20.0 ± 7.1* (n = 5) | 36.5 ± 9.0 | 37.3 ± 9.0 |
| IL-2 | 7.2 ± 2.9 (n = 4) | 8.6 ± 1.7 (n = 5) | 22.7 ± 7.7 | 23.7 ± 6.2 |
| IL-4 | 2.1 ± 0.8 (n = 5) | 2.5 ± 0.3 (n = 5) | 1.2 ± 0.5 | 2.0 ± 0.8 |
| Perforin | 4.0 ± 1.7 (n = 14) | 12.1 ± 4.2* (n = 11) | 0.4 ± 0.2 | 2.0 ± 0.5† |