HUBEC coculture promotes the expansion of human CD34+ subsets and CFCs compared with controls
| Conditions . | Cell yield . | No. cells procured × 105 . | No. CFCs × 104 . | Total . | ||||
|---|---|---|---|---|---|---|---|---|
| CD34+ . | CD34+CD38+(%) . | CD34+CD38−(%) . | CFU-GMs . | BFU-Es . | CFU-Mix's . | |||
| Input | 5.0 | 5.0 | 4.91 ± 0.07 (98.4) | 0.08 ± 0.04 (1.6) | 3.7 ± 0.9 | 0.6 ± 0.3 | 0.5 ± 0.2 | 4.7 ± 1.4 |
| HUBECs | 81.0 ± 3.4 (16.1-fold) | 27.0 ± 1.1 (5.4-fold) | 9.0 ± 0.4 (12.3) | 17.0 ± 0.5 (21.6) | 56.0 ± 2.2 | 4.8 ± 0.6 | 2.6 ± 0.3 | 63.4 ± 2.5 |
| HUBECs, noncontact | 82.0 ± 3.7 (16.4-fold) | 14.0 ± 0.1 (2.8-fold) | 10.4 ± 0.3 (13.0) | 2.8 ± 0.1 (3.4) | 30.0 ± 1.3 | 6.0 ± 0.5 | 4.3 ± 0.9 | 40.3 ± 0.5 |
| Stroma-free | 51.0 ± 1.3 (10.2-fold) | 15.1 ± 0.7 (3.0-fold) | 15.1 ± 0.6 (29.6) | 0.02 ± 0.02 (0.1) | 15.8 ± 4.5 | 0.7 ± 0.7 | 0.5 ± 0.9 | 17.1 ± 4.6 |
| Nonbrain ECs | 52.0 ± 2.8 (10.4-fold) | 18.1 ± 12.6 (3.6-fold) | 18.1 ± 12.6 (34.8) | 0 (0) | 22.2 ± 4.9 | 4.8 ± 0.3 | 0.6 ± 0.3 | 27.7 ± 6.2 |
| Conditions . | Cell yield . | No. cells procured × 105 . | No. CFCs × 104 . | Total . | ||||
|---|---|---|---|---|---|---|---|---|
| CD34+ . | CD34+CD38+(%) . | CD34+CD38−(%) . | CFU-GMs . | BFU-Es . | CFU-Mix's . | |||
| Input | 5.0 | 5.0 | 4.91 ± 0.07 (98.4) | 0.08 ± 0.04 (1.6) | 3.7 ± 0.9 | 0.6 ± 0.3 | 0.5 ± 0.2 | 4.7 ± 1.4 |
| HUBECs | 81.0 ± 3.4 (16.1-fold) | 27.0 ± 1.1 (5.4-fold) | 9.0 ± 0.4 (12.3) | 17.0 ± 0.5 (21.6) | 56.0 ± 2.2 | 4.8 ± 0.6 | 2.6 ± 0.3 | 63.4 ± 2.5 |
| HUBECs, noncontact | 82.0 ± 3.7 (16.4-fold) | 14.0 ± 0.1 (2.8-fold) | 10.4 ± 0.3 (13.0) | 2.8 ± 0.1 (3.4) | 30.0 ± 1.3 | 6.0 ± 0.5 | 4.3 ± 0.9 | 40.3 ± 0.5 |
| Stroma-free | 51.0 ± 1.3 (10.2-fold) | 15.1 ± 0.7 (3.0-fold) | 15.1 ± 0.6 (29.6) | 0.02 ± 0.02 (0.1) | 15.8 ± 4.5 | 0.7 ± 0.7 | 0.5 ± 0.9 | 17.1 ± 4.6 |
| Nonbrain ECs | 52.0 ± 2.8 (10.4-fold) | 18.1 ± 12.6 (3.6-fold) | 18.1 ± 12.6 (34.8) | 0 (0) | 22.2 ± 4.9 | 4.8 ± 0.3 | 0.6 ± 0.3 | 27.7 ± 6.2 |
Purified human ABM CD34+ cells (5 × 105) were plated per culture treatment (n = 8) as described in “Materials and methods.” Nonadherent cells were harvested on day 7 of culture and analyzed via flow cytometry. Numbers in parentheses in the cell-yield and CD34+ columns reflect the fold-increase in these populations following culture. Numbers in parentheses in the CD34+ CD38+ and CD34+CD38− columns indicate the percentage of each population as a subset of the total population. CFC values represent the total number of CFC per culture.
CFU-GMs indicates GM colony-forming units; BFU-Es, erythroid burst-forming units; CFU-Mix's, mixed CFUs.