HUBEC-cultured cells generate multilineage human cell differentiation and human progenitor cell colonies within the marrow of NOD/SCID mice that underwent transplantation
| Culture conditions . | Human CFCs formed from ABM of NOD/SCID mice . | Percentage of engrafted CD45+ cells expressing specified CD cells . | |||||
|---|---|---|---|---|---|---|---|
| GM . | E . | Mix . | Total . | CD34 . | CD19 . | CD13 . | |
| Fresh ABM | |||||||
| CD34+cells | 0.8 ± 0.5 | 0.2 ± 0.2 | 0.2 ± 0.2 | 1.2 ± 0.8 | 28.2 ± 3.6 | 89.9 ± 4.1 | 9.5 ± 3.4 |
| HUBECs | 44.2 ± 15.9 | 3.4 ± 1.7 | 1.6 ± 1.5 | 49.0 ± 18.5 | 32.3 ± 9.4 | 77.7 ± 16.7 | 19.3 ± 7.7 |
| Culture conditions . | Human CFCs formed from ABM of NOD/SCID mice . | Percentage of engrafted CD45+ cells expressing specified CD cells . | |||||
|---|---|---|---|---|---|---|---|
| GM . | E . | Mix . | Total . | CD34 . | CD19 . | CD13 . | |
| Fresh ABM | |||||||
| CD34+cells | 0.8 ± 0.5 | 0.2 ± 0.2 | 0.2 ± 0.2 | 1.2 ± 0.8 | 28.2 ± 3.6 | 89.9 ± 4.1 | 9.5 ± 3.4 |
| HUBECs | 44.2 ± 15.9 | 3.4 ± 1.7 | 1.6 ± 1.5 | 49.0 ± 18.5 | 32.3 ± 9.4 | 77.7 ± 16.7 | 19.3 ± 7.7 |
NOD/SCID mice received transplants of either 1 × 106 ABM CD34+ cells or the progeny of this dose of ABM CD34+ cells following 7 days of HUBEC culture as described in “Materials and methods.” The CFC content in each group was determined from triplicate cultures of 1 × 105 NOD/SCID marrow cells per dish under procedures described in “Materials and methods” (n = 11). The numbers shown under the CD34, CD19, and CD13 columns indicate the percentage of engrafted human CD45+ cells that expressed the particular differentiation antigen. For animals receiving transplants of fresh ABM CD34+ cells, we were able to analyze only those mice with human cell engraftment ≥ 1%.