Table 1.

Effect of antisense Mpl expression on the formation of CJ7 EBs

CJ7MSCVMPL-AS1MPL-AS2MPL-AS3
Total EBs 334 362 384 533 85  
Hematopoietic EBs 59 47 32 10 
CJ7MSCVMPL-AS1MPL-AS2MPL-AS3
Total EBs 334 362 384 533 85  
Hematopoietic EBs 59 47 32 10 

Total number of embryoid bodies obtained after 12 days in culture. Two thousand CJ7 cells per dish were mixed with 15 000 MS-5 cells and cultured in methylcellulose using the differentiation medium described in “Materials and methods.” EBs containing red cells are scored as hematopoietic EBs.

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