Effect of antisense Mpl expression on the formation of CJ7 EBs
.
CJ7
.
MSCV
.
MPL-AS1
.
MPL-AS2
.
MPL-AS3
.
Total EBs
334
362
384
533
85
Hematopoietic EBs
59
47
6
32
10
.
CJ7
.
MSCV
.
MPL-AS1
.
MPL-AS2
.
MPL-AS3
.
Total EBs
334
362
384
533
85
Hematopoietic EBs
59
47
6
32
10
Total number of embryoid bodies obtained after 12 days in culture. Two thousand CJ7 cells per dish were mixed with 15 000 MS-5 cells and cultured in methylcellulose using the differentiation medium described in “Materials and methods.” EBs containing red cells are scored as hematopoietic EBs.