Kinetic parameters for the interaction of GST-SH2-SHIP with synthetic peptides
| Peptides . | kon (M−1s−1) . | koff (ms−1) . | KD (nM) . |
|---|---|---|---|
| P1 | NM | NM | NM |
| P2 | 1295 | 0.181 | 149.0 |
| P3 | 1709 | 0.125 | 75.1 |
| P4 | 2616 | 0.188 | 71.0 |
| pITIM | 3370 | 0.154 | 47.2 |
| pIg-α | 1285 | 0.520 | 402.0 |
| Peptides . | kon (M−1s−1) . | koff (ms−1) . | KD (nM) . |
|---|---|---|---|
| P1 | NM | NM | NM |
| P2 | 1295 | 0.181 | 149.0 |
| P3 | 1709 | 0.125 | 75.1 |
| P4 | 2616 | 0.188 | 71.0 |
| pITIM | 3370 | 0.154 | 47.2 |
| pIg-α | 1285 | 0.520 | 402.0 |
The measurements were performed at 30 μL/min of flow rate at 25°C. The chip was regenerated by washing with phosphate buffered saline containing 0.05% sodium dodecyl sulfate. The data were calculated from at least 5 different concentrations by BIAevaluation software. NM indicates not measurable.