Table 4. MCL patients using the V H 3-21... | American Society of Hematology

Table 4.

MCL patients using the V_H3-21 gene

MCL patient	Morphology	Light-chain expression	V_Hgene homology, %	N-D-N	D	J_H	V_H gene frame	V_λ	J_λ
5	MCL	λ	100	AGGAGG-GTATTACGATTTTTGGAGTGGTTATTAT-TCTTGG	D3-3	J_H6	IF	V_λ3-19	J_λ2/3
14^4-150	MCL	λ	99.6	GATCCGAC-CAGCAGCTGG-GACGTAACACCCAAAT	D6-13	J_H4	IF	na	na
23	MCL	λ	99.1	GA-AGTAGTACCAGCTGTTATA-GACATA	D2-2	J_H6	IF	OF^‡	OF^‡
29	MCL	λ	100	GAAATGTCAGG-CGATTTTTGGAGTGGTTAT-A	D3-3	J_H6	IF	nt	nt
32	MCL	λ	98.6	GAA-TATAGCAGCAGCT-CCA	D6-13	J_H6	IF	V_λ3-19	J_λ2/3
33	MCL	λ	100	GATTTCTCGGCCGAGTCT-TGGTTCGGGGAGTTATTAT-CTAG	D3-10	J_H6	IF	V_λ3-19	J_λ3
34	MCL-BV	λ	99.6	GGACTCTGG-TGGAGTGGTTATTATA-G	D3-3	J_H6	IF	V_λ3-19	J_λ2/3
41^4-150	MCL	λ	99.6	GATCTCACGGGCTTCGCCCTG	ND	J_H6	IF	V_λ3-19	J_λ3
54	MCL	λ	100	AAGAATCACGATTTTCT-TTTGGGG-CACTGGG	D3-16	J_H6	IF	V_λ3-19^4-151	J_λ2/3
58	MCL-BV	κ	100	TAGGATCATAC-TAGCAGCT-TCT	D6-6	J_H5	SC	na	na
66	MCL-BV	λ	100	GAAAGC-ACTATGATAGTAGTGGTTA-CCCAGGCGC	D3-22	J_H2	IF	V_λ3-19	J_λ2/3
68	MCL	λ	98.7	GGGGAGAAT-TATTACGATATTTTCACTGGTTAT-CACACAAC	D3-9	J_H4	IF	V_λ9-49	J_λ2/3
72	MCL-BV	λ	100	GCCCTTAATCACGGGGGGTGGGAA	ND	J_H6	IF	V_λ3-19	J_λ1
74	MCL-BV	λ	98.7	GATACGACATACCTGCTATGGGGGATTATGAT-GTATTACT	D3-22	J_H4	IF	V_λ3-19	J_λ2/3
86	MCL-BV	λ	99.1	GATAGT-CAGTGGCTGG-AAAT	D6-19	J_H6	IF	V_λ3-19	J_λ3
91	MCL	λ	99.6	GATCAGC-TACTATGGTTCGGGGAGTTATTA-CAAGCCCAGGG	D3-10	J_H6	IF	V_λ3-19	J_λ2/3
92	MCL	λ	99.6	GATCCT-GTATTACGATATTTTGACTGG-CTAT	D3-3	J_H3	SC	V_λ3-19	J_λ3
97	MCL	λ	99.1	GAGAATCCCCGCCCGG-TTGTGGTGGTGATTGCTA-CCCAT	D2-15	J_H3	IF	V_λ3-19^4-151	J_λ2/3
98	MCL-BV	λ	99.6	GATA-ATAGTGGCTACGA-CCTACGAGG	D5-12	J_H6	IF	V_λ3-19	J_λ2/3
103	MCL	λ	99.6	GAACCT-TACCAGCTGCTAT-TGGGA	D2-2	J_H6	IF	V_λ3-19	J_λ2/3
105	MCL	λ	100	GAAGG-TTACGATTTTTGGAGTGGTTATT-CAA	D3-3	J_H6	IF	V_λ3-19	J_λ2/3

MCL patient	Morphology	Light-chain expression	V_Hgene homology, %	N-D-N	D	J_H	V_H gene frame	V_λ	J_λ
5	MCL	λ	100	AGGAGG-GTATTACGATTTTTGGAGTGGTTATTAT-TCTTGG	D3-3	J_H6	IF	V_λ3-19	J_λ2/3
14^4-150	MCL	λ	99.6	GATCCGAC-CAGCAGCTGG-GACGTAACACCCAAAT	D6-13	J_H4	IF	na	na
23	MCL	λ	99.1	GA-AGTAGTACCAGCTGTTATA-GACATA	D2-2	J_H6	IF	OF^‡	OF^‡
29	MCL	λ	100	GAAATGTCAGG-CGATTTTTGGAGTGGTTAT-A	D3-3	J_H6	IF	nt	nt
32	MCL	λ	98.6	GAA-TATAGCAGCAGCT-CCA	D6-13	J_H6	IF	V_λ3-19	J_λ2/3
33	MCL	λ	100	GATTTCTCGGCCGAGTCT-TGGTTCGGGGAGTTATTAT-CTAG	D3-10	J_H6	IF	V_λ3-19	J_λ3
34	MCL-BV	λ	99.6	GGACTCTGG-TGGAGTGGTTATTATA-G	D3-3	J_H6	IF	V_λ3-19	J_λ2/3
41^4-150	MCL	λ	99.6	GATCTCACGGGCTTCGCCCTG	ND	J_H6	IF	V_λ3-19	J_λ3
54	MCL	λ	100	AAGAATCACGATTTTCT-TTTGGGG-CACTGGG	D3-16	J_H6	IF	V_λ3-19^4-151	J_λ2/3
58	MCL-BV	κ	100	TAGGATCATAC-TAGCAGCT-TCT	D6-6	J_H5	SC	na	na
66	MCL-BV	λ	100	GAAAGC-ACTATGATAGTAGTGGTTA-CCCAGGCGC	D3-22	J_H2	IF	V_λ3-19	J_λ2/3
68	MCL	λ	98.7	GGGGAGAAT-TATTACGATATTTTCACTGGTTAT-CACACAAC	D3-9	J_H4	IF	V_λ9-49	J_λ2/3
72	MCL-BV	λ	100	GCCCTTAATCACGGGGGGTGGGAA	ND	J_H6	IF	V_λ3-19	J_λ1
74	MCL-BV	λ	98.7	GATACGACATACCTGCTATGGGGGATTATGAT-GTATTACT	D3-22	J_H4	IF	V_λ3-19	J_λ2/3
86	MCL-BV	λ	99.1	GATAGT-CAGTGGCTGG-AAAT	D6-19	J_H6	IF	V_λ3-19	J_λ3
91	MCL	λ	99.6	GATCAGC-TACTATGGTTCGGGGAGTTATTA-CAAGCCCAGGG	D3-10	J_H6	IF	V_λ3-19	J_λ2/3
92	MCL	λ	99.6	GATCCT-GTATTACGATATTTTGACTGG-CTAT	D3-3	J_H3	SC	V_λ3-19	J_λ3
97	MCL	λ	99.1	GAGAATCCCCGCCCGG-TTGTGGTGGTGATTGCTA-CCCAT	D2-15	J_H3	IF	V_λ3-19^4-151	J_λ2/3
98	MCL-BV	λ	99.6	GATA-ATAGTGGCTACGA-CCTACGAGG	D5-12	J_H6	IF	V_λ3-19	J_λ2/3
103	MCL	λ	99.6	GAACCT-TACCAGCTGCTAT-TGGGA	D2-2	J_H6	IF	V_λ3-19	J_λ2/3
105	MCL	λ	100	GAAGG-TTACGATTTTTGGAGTGGTTATT-CAA	D3-3	J_H6	IF	V_λ3-19	J_λ2/3

F4-150

Second V_H gene rearrangement was also detected

MCL-BV indicates blastoid variant; N, N-nucleotides.

F4-151

Second out-of-frame rearrangement was also detected.

‡

OF, 2 out-of-frame V_λ gene rearrangements detected (V_λ7-43/J_λ1 and V_λ2-23/J_λ2).

IF, in-frame rearrangement; SC, stop codon in rearrangement, but no other IgH rearrangement was detected; na, not amplifiable; nt, insufficient DNA remaining and therefore not tested; and ND, not able to define.

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