Hemogenic ability of VE cadherin+(CD45− Ter119−) cells obtained from transgenic embryos
| Embryo . | No. of cells/well . | No. of positive wells/no. of wells analyzed . | |||||
|---|---|---|---|---|---|---|---|
| VE cadherin+GFP− . | VE cadherin+GFP+ . | ||||||
| HPC colonies . | EC colonies . | Both . | HPC colonies . | EC colonies . | Both . | ||
| 10.5-dpc | |||||||
| Exp 1 | 30 | 2/6 | 4/6 | 1/6 | 0/6 | 4/6 | 0/6 |
| Exp 2 | 50 | 4/6 | 4/6 | 3/6 | 0/6 | 6/6 | 0/6 |
| 11.5-dpc | |||||||
| Exp 1 | 15 | 2/4 | 2/4 | 1/4 | 0/4 | 3/4 | 0/4 |
| Exp 2 | 30 | 9/12 | 10/12 | 8/12 | 0/9 | 8/9 | 0/9 |
| Embryo . | No. of cells/well . | No. of positive wells/no. of wells analyzed . | |||||
|---|---|---|---|---|---|---|---|
| VE cadherin+GFP− . | VE cadherin+GFP+ . | ||||||
| HPC colonies . | EC colonies . | Both . | HPC colonies . | EC colonies . | Both . | ||
| 10.5-dpc | |||||||
| Exp 1 | 30 | 2/6 | 4/6 | 1/6 | 0/6 | 4/6 | 0/6 |
| Exp 2 | 50 | 4/6 | 4/6 | 3/6 | 0/6 | 6/6 | 0/6 |
| 11.5-dpc | |||||||
| Exp 1 | 15 | 2/4 | 2/4 | 1/4 | 0/4 | 3/4 | 0/4 |
| Exp 2 | 30 | 9/12 | 10/12 | 8/12 | 0/9 | 8/9 | 0/9 |
Indicated numbers of cells were sorted from embryos transgenic for GFP gene under the control of Flk-1 promoter/ enhancer and cocultured with OP9 stromal cells with SCF, IL-3, Epo, and G-CSF for 10 days. Existences of hematopoietic colonies were judged by their morphology, and those of endothelial colonies were by immunostaining with anti-PECAM-I MoAb. Exp, experiment.