Comparison of thrombins ability to activate FVIII and FV
| Mutation* . | Location† . | FV activation (% WT activation)‡ . | FVIII activation (% WT activation) . | FVIII activation (from DRC)1-153 . |
|---|---|---|---|---|
| Lys21Ala | ABE-I | 38.1 ± 3.5 | 46.0 ± 12.5 | — |
| Lys65Ala | ABE-I | 46.2 ± 4.6 | 8.5 ± 6.5 | 11.5 ± 2.1 |
| His66Ala | ABE-I | 30.5 ± 0.3 | 25.1 ± 1.0 | — |
| Arg68Ala | ABE-I | 12.5 ± 1.9 | 10.4 ± 12.3 | 7.5 ± 0.7 |
| Arg70Ala | ABE-I | 26.9 ± 3.6 | 35.8 ± 3.6 | — |
| Tyr71Ala | ABE-I | 6.2 ± 4.1 | 3.3 ± 5.7 | 6.0 ± 0.1 |
| Arg73Ala | ABE-I | 37.8 ± 0.8 | 70.7 ± 2.3 | — |
| Lys77Ala | ABE-I | 36.0 ± 0.3 | 112.4 ± 21.1 | — |
| Trp50Ala | 50-loop | 28.6 ± 9.1 | 8.9 ± 7.8 | 5.8 ± 1.1 |
| Lys52Ala | 50-loop | 28.8 ± 4.0 | 57.9 ± 15.6 | — |
| Arg89Ala/Arg93Ala/Glu94Ala | ABE-II | 39.7 ± 12.1 | 66.7 ± 4.4 | — |
| Arg98Ala | ABE-II | 27.0 ± 4.2 | 43.3 ± 5.7 | 32.4 ± 8.8 |
| Arg245Ala/Lys248Ala/Gln251Ala | ABE-II | 45.7 ± 5.4 | 65.1 ± 7.3 | — |
| Glu229Ala | Na+ site | 5.2 ± 5.0 | 2.9 ± 2.1 | 4.3 ± 0.4 |
| Arg233Ala | Na+ site | 15.4 ± 3.7 | 11.5 ± 2.6 | — |
| Asp234Ala | Na+ site | 31.9 ± 16.4 | 31.6 ± 17.6 | — |
| Asp193Ala/Lys196Ala | Na+ site | 54.2 ± 1.9 | 35.6 ± 9.1 | — |
| Mutation* . | Location† . | FV activation (% WT activation)‡ . | FVIII activation (% WT activation) . | FVIII activation (from DRC)1-153 . |
|---|---|---|---|---|
| Lys21Ala | ABE-I | 38.1 ± 3.5 | 46.0 ± 12.5 | — |
| Lys65Ala | ABE-I | 46.2 ± 4.6 | 8.5 ± 6.5 | 11.5 ± 2.1 |
| His66Ala | ABE-I | 30.5 ± 0.3 | 25.1 ± 1.0 | — |
| Arg68Ala | ABE-I | 12.5 ± 1.9 | 10.4 ± 12.3 | 7.5 ± 0.7 |
| Arg70Ala | ABE-I | 26.9 ± 3.6 | 35.8 ± 3.6 | — |
| Tyr71Ala | ABE-I | 6.2 ± 4.1 | 3.3 ± 5.7 | 6.0 ± 0.1 |
| Arg73Ala | ABE-I | 37.8 ± 0.8 | 70.7 ± 2.3 | — |
| Lys77Ala | ABE-I | 36.0 ± 0.3 | 112.4 ± 21.1 | — |
| Trp50Ala | 50-loop | 28.6 ± 9.1 | 8.9 ± 7.8 | 5.8 ± 1.1 |
| Lys52Ala | 50-loop | 28.8 ± 4.0 | 57.9 ± 15.6 | — |
| Arg89Ala/Arg93Ala/Glu94Ala | ABE-II | 39.7 ± 12.1 | 66.7 ± 4.4 | — |
| Arg98Ala | ABE-II | 27.0 ± 4.2 | 43.3 ± 5.7 | 32.4 ± 8.8 |
| Arg245Ala/Lys248Ala/Gln251Ala | ABE-II | 45.7 ± 5.4 | 65.1 ± 7.3 | — |
| Glu229Ala | Na+ site | 5.2 ± 5.0 | 2.9 ± 2.1 | 4.3 ± 0.4 |
| Arg233Ala | Na+ site | 15.4 ± 3.7 | 11.5 ± 2.6 | — |
| Asp234Ala | Na+ site | 31.9 ± 16.4 | 31.6 ± 17.6 | — |
| Asp193Ala/Lys196Ala | Na+ site | 54.2 ± 1.9 | 35.6 ± 9.1 | — |