Real-time quantitative PCR analysis of KGF cDNA
| . | HPRT (Ct) . | KGF (Ct) . | ΔCt . |
|---|---|---|---|
| Whole thymus | 25.08 | 33.59 | 8.51 |
| Thymocyte | 25.01 | 32.05 | 7.04 |
| RAG 2−/−thymocyte | 28.07 | Not detected | — |
| CD3−/4−/8−thymocyte | 24.75 ± 0.52 | Not detected | — |
| CD4+/8+thymocyte | 25.45 ± 0.18 | 32.58 ± 0.08 | 7.13 |
| CD4+/8−thymocyte | 27.43 ± 0.78 | 33.43 ± 0.76 | 6.00 |
| . | HPRT (Ct) . | KGF (Ct) . | ΔCt . |
|---|---|---|---|
| Whole thymus | 25.08 | 33.59 | 8.51 |
| Thymocyte | 25.01 | 32.05 | 7.04 |
| RAG 2−/−thymocyte | 28.07 | Not detected | — |
| CD3−/4−/8−thymocyte | 24.75 ± 0.52 | Not detected | — |
| CD4+/8+thymocyte | 25.45 ± 0.18 | 32.58 ± 0.08 | 7.13 |
| CD4+/8−thymocyte | 27.43 ± 0.78 | 33.43 ± 0.76 | 6.00 |
A 10-fold alteration in message level of KGF or HPRT is reflected by ΔCt of 3.939 and 3.936, respectively.
— indicates not applicable.