Cell cycle profile and distribution
| . | Sub-G1 . | G0G1 . | S . | G2/M . |
|---|---|---|---|---|
| MM.1S cells (%) | ||||
| Control | 6.0 | 46.5 | 32.1 | 15.4 |
| 24 h | 70.1 | 22.1 | 6.5 | 1.2 |
| 48 h | 91.4 | 5.9 | 2.0 | 0.7 |
| 72 h | 100.0 | 0.0 | 0.0 | 0.0 |
| 96 h | 100.0 | 0.0 | 0.0 | 0.0 |
| MM.1R cells (%) | ||||
| Control | 4.1 | 42.1 | 22.4 | 31.4 |
| 24 h | 50.9 | 26.2 | 11.3 | 11.3 |
| 48 h | 90.4 | 4.6 | 5.0 | 0.0 |
| 72 h | 100.0 | 0.0 | 0.0 | 0.0 |
| 96 h | 100.0 | 0.0 | 0.0 | 0.0 |
| RPMI-8226/S cells (%) | ||||
| Control | 4.8 | 20.9 | 38.8 | 35.5 |
| 24 h | 55.0 | 12.5 | 0.4 | 32.1 |
| 48 h | 98.2 | 0.4 | 0.0 | 1.4 |
| 72 h | 95.9 | 0.9 | 0.3 | 2.8 |
| 96 h | 100.0 | 0.0 | 0.0 | 0.0 |
| RPMI-8226-Dox40 cells (%) | ||||
| Control | 5.3 | 20.8 | 35.6 | 38.3 |
| 24 h | 58.6 | 9.9 | 5.0 | 26.4 |
| 48 h | 86.0 | 1.3 | 12.7 | 0.1 |
| 72 h | 100.0 | 0.0 | 0.0 | 0.0 |
| 96 h | 99.0 | 0.0 | 0.9 | 0.1 |
| RPMI-8226-LR5 cells (%) | ||||
| Control | 5.6 | 27.5 | 66.9 | 0.0 |
| 24 h | 29.0 | 28.5 | 0.0 | 42.5 |
| 48 h | 71.8 | 8.8 | 0.0 | 19.5 |
| 72 h | 99.3 | 0.1 | 0.0 | 0.6 |
| ARP-1 cells (%) | ||||
| Control | 4.5 | 25.0 | 70.4 | 0.1 |
| 24 h | 16.3 | 17.4 | 4.6 | 61.7 |
| 48 h | 95.0 | 2.9 | 0.0 | 2.1 |
| 72 h | 96.0 | 2.4 | 0.0 | 1.6 |
| LP-1 cells (%) | ||||
| Control | 0.0 | 42.6 | 55.0 | 2.4 |
| 24 h | 4.5 | 3.7 | 0.0 | 91.8 |
| 48 h | 61.3 | 1.0 | 2.7 | 35.1 |
| 72 h | 60.3 | 22.7 | 0.0 | 17.1 |
| ARH-77 cells (%) | ||||
| Control | 3.6 | 62.9 | 29.6 | 4.0 |
| 24 h | 12.5 | 40.2 | 25.3 | 22.1 |
| 48 h | 40.5 | 27.3 | 14.4 | 17.8 |
| 72 h | 92.4 | 0.0 | 0.0 | 7.6 |
| 96 h | 95.3 | 0.0 | 0.2 | 4.5 |
| IM-9 cells (%) | ||||
| Control | 0.5 | 44.2 | 40.7 | 14.3 |
| 24 h | 16.3 | 36.6 | 13.2 | 33.8 |
| 48 h | 2.9 | 63.3 | 29.8 | 4.0 |
| 72 h | 99.7 | 0.0 | 0.0 | 0.3 |
| 96 h | 99.5 | 0.0 | 0.0 | 0.5 |
| . | Sub-G1 . | G0G1 . | S . | G2/M . |
|---|---|---|---|---|
| MM.1S cells (%) | ||||
| Control | 6.0 | 46.5 | 32.1 | 15.4 |
| 24 h | 70.1 | 22.1 | 6.5 | 1.2 |
| 48 h | 91.4 | 5.9 | 2.0 | 0.7 |
| 72 h | 100.0 | 0.0 | 0.0 | 0.0 |
| 96 h | 100.0 | 0.0 | 0.0 | 0.0 |
| MM.1R cells (%) | ||||
| Control | 4.1 | 42.1 | 22.4 | 31.4 |
| 24 h | 50.9 | 26.2 | 11.3 | 11.3 |
| 48 h | 90.4 | 4.6 | 5.0 | 0.0 |
| 72 h | 100.0 | 0.0 | 0.0 | 0.0 |
| 96 h | 100.0 | 0.0 | 0.0 | 0.0 |
| RPMI-8226/S cells (%) | ||||
| Control | 4.8 | 20.9 | 38.8 | 35.5 |
| 24 h | 55.0 | 12.5 | 0.4 | 32.1 |
| 48 h | 98.2 | 0.4 | 0.0 | 1.4 |
| 72 h | 95.9 | 0.9 | 0.3 | 2.8 |
| 96 h | 100.0 | 0.0 | 0.0 | 0.0 |
| RPMI-8226-Dox40 cells (%) | ||||
| Control | 5.3 | 20.8 | 35.6 | 38.3 |
| 24 h | 58.6 | 9.9 | 5.0 | 26.4 |
| 48 h | 86.0 | 1.3 | 12.7 | 0.1 |
| 72 h | 100.0 | 0.0 | 0.0 | 0.0 |
| 96 h | 99.0 | 0.0 | 0.9 | 0.1 |
| RPMI-8226-LR5 cells (%) | ||||
| Control | 5.6 | 27.5 | 66.9 | 0.0 |
| 24 h | 29.0 | 28.5 | 0.0 | 42.5 |
| 48 h | 71.8 | 8.8 | 0.0 | 19.5 |
| 72 h | 99.3 | 0.1 | 0.0 | 0.6 |
| ARP-1 cells (%) | ||||
| Control | 4.5 | 25.0 | 70.4 | 0.1 |
| 24 h | 16.3 | 17.4 | 4.6 | 61.7 |
| 48 h | 95.0 | 2.9 | 0.0 | 2.1 |
| 72 h | 96.0 | 2.4 | 0.0 | 1.6 |
| LP-1 cells (%) | ||||
| Control | 0.0 | 42.6 | 55.0 | 2.4 |
| 24 h | 4.5 | 3.7 | 0.0 | 91.8 |
| 48 h | 61.3 | 1.0 | 2.7 | 35.1 |
| 72 h | 60.3 | 22.7 | 0.0 | 17.1 |
| ARH-77 cells (%) | ||||
| Control | 3.6 | 62.9 | 29.6 | 4.0 |
| 24 h | 12.5 | 40.2 | 25.3 | 22.1 |
| 48 h | 40.5 | 27.3 | 14.4 | 17.8 |
| 72 h | 92.4 | 0.0 | 0.0 | 7.6 |
| 96 h | 95.3 | 0.0 | 0.2 | 4.5 |
| IM-9 cells (%) | ||||
| Control | 0.5 | 44.2 | 40.7 | 14.3 |
| 24 h | 16.3 | 36.6 | 13.2 | 33.8 |
| 48 h | 2.9 | 63.3 | 29.8 | 4.0 |
| 72 h | 99.7 | 0.0 | 0.0 | 0.3 |
| 96 h | 99.5 | 0.0 | 0.0 | 0.5 |
Cell cycle profile and distribution were defined by PI analysis of cells that were either treated with SAHA (5 μM) in 10% FCS for 24, 48, 72, or 96 hours or untreated controls. The percentage of cells in the sub-Gt region indicates significant SAHA-induced cell death.