Adult human BM contains B-lymphocyte precursors revealed by transplantation in immunodeficient NOD/SCID mice
| Cells injected . | No. of cells Injected . | No. of CD34+CD38− cells injected* . | No. of engrafted/injected mice (%) . | Percentage of hCD45+CD19+ cells in BM of chimeras . |
|---|---|---|---|---|
| Fetal BM CD34+ | 2.9-3 × 106 | 28.5-30 × 103 | 5/6 (83) | 1.54 (± 1.28) |
| CB CD34+ | 5 × 105 | 4 × 103 | 11/11 (100) | 1.02 (± 0.86) |
| Adult BM CD34+ | 2 × 106 | 39.6-72 × 103 | 5/5 (100) | 0.22 (± 0.16) |
| CB CD34− | 5 × 106 | <0.01 | 0/5 | <0.02 |
| Cells injected . | No. of cells Injected . | No. of CD34+CD38− cells injected* . | No. of engrafted/injected mice (%) . | Percentage of hCD45+CD19+ cells in BM of chimeras . |
|---|---|---|---|---|
| Fetal BM CD34+ | 2.9-3 × 106 | 28.5-30 × 103 | 5/6 (83) | 1.54 (± 1.28) |
| CB CD34+ | 5 × 105 | 4 × 103 | 11/11 (100) | 1.02 (± 0.86) |
| Adult BM CD34+ | 2 × 106 | 39.6-72 × 103 | 5/5 (100) | 0.22 (± 0.16) |
| CB CD34− | 5 × 106 | <0.01 | 0/5 | <0.02 |
Numbers of CD34+CD38− cells were determined by flow cytometry, using small aliquots of each preparation of CD34+ enriched cells. These results were obtained in 2 very similar but independent experiments.