Primers for site-directed mutagenesis of the P2Y12 receptor
| Amino acid and position . | Nucleotide change . | Amino acid change . | Sense . | Primers used . |
|---|---|---|---|---|
| Cys17 | TGC>TCC | Cys→Ser | + | 5′ -GGAACACCAGTCTGTCCACCAGAGACTAC-3′ |
| − | 5′ -GTAGTCTCTGGTGGACAGACTGGTGTTCC-3′ | |||
| Cys97 | TGT>TCT | Cys→Ser | + | 5′ -GGACCACTGAGAACTTTTGTGTCTCAAGTTACCTCCGTC-3′ |
| − | 5′ -GACGGAGGTAACTTGAGACACAAAAGTTCTCAGTGGTCC-3′ | |||
| Cys175 | TGC>TCC | Cys→Ser | + | 5′ -GCCGAGAGACAAGAATGTGAAGAAATCCTCTTTCCTTAAATC-3′ |
| − | 5′ -GATTTAAGGAAAGAGGATTTCTTCACATTCTTGTCTCTCGGC-3′ | |||
| Cys270 | TGC>TCC | Cys→Ser | + | 5′ -CCCGGGATGTCTTTGACTCCACTGCTGAAAATAC-3′ |
| − | 5′ -GTATTTTCAGCAGTGGAGTCAAAGACATCCCGGG-3′ |
| Amino acid and position . | Nucleotide change . | Amino acid change . | Sense . | Primers used . |
|---|---|---|---|---|
| Cys17 | TGC>TCC | Cys→Ser | + | 5′ -GGAACACCAGTCTGTCCACCAGAGACTAC-3′ |
| − | 5′ -GTAGTCTCTGGTGGACAGACTGGTGTTCC-3′ | |||
| Cys97 | TGT>TCT | Cys→Ser | + | 5′ -GGACCACTGAGAACTTTTGTGTCTCAAGTTACCTCCGTC-3′ |
| − | 5′ -GACGGAGGTAACTTGAGACACAAAAGTTCTCAGTGGTCC-3′ | |||
| Cys175 | TGC>TCC | Cys→Ser | + | 5′ -GCCGAGAGACAAGAATGTGAAGAAATCCTCTTTCCTTAAATC-3′ |
| − | 5′ -GATTTAAGGAAAGAGGATTTCTTCACATTCTTGTCTCTCGGC-3′ | |||
| Cys270 | TGC>TCC | Cys→Ser | + | 5′ -CCCGGGATGTCTTTGACTCCACTGCTGAAAATAC-3′ |
| − | 5′ -GTATTTTCAGCAGTGGAGTCAAAGACATCCCGGG-3′ |
Underlined nucleotide indicates the altered nucleotide for site-directed mutagenesis.