Cytogenetic, cytochemical, and immunophenotypic characteristics of myeloid leukemias
| Sample . | FAB subtype . | Immunophenotype* . | Cytogenetic phenotype . | Cytochemistry . | ||
|---|---|---|---|---|---|---|
| Per . | NSE . | PAS . | ||||
| 1 | M0 | CD13, CD34, CD45, HLA-DR | NL | <3% | − | − |
| 2 | M1 | CD33, CD45, HLA-DR, TdT | NL | <10% | − | − |
| 3 | M1 | CD5, CD7, CD11b, CD13, HLA-DR, CD45 | NL | 20% | − | − |
| 4 | M1 | CD13, CD33, CD45 | NL | + | − | − |
| 5 | M1 | ND | ND | <10% | − | − |
| 6 | M2 | CD13, CD15, CD34, CD45, TdT | NL | + | − | − |
| 7 | M3 | CD2, CD13, CD33, CD45 | t(15:17) | + | + | − |
| 8 | M4 | CD33, CD45 | inv(16)(p32q32) | + | − | − |
| 9 | M5 | ND | ND | ND | ND | ND |
| 10 | M5a | ND | ND | + | + | − |
| 11 | M5b | CD5, CD11b, CD13, CD14, CD15, CD24, CD45 | ND | + | + | + |
| 12 | CML-B/M2 | CD2, CD13, CD33 CD34, CD38, CD45, HLA-DR | 47XY t(9:22) | + | − | − |
| 13 | CML | ND | NL | + | + | + |
| Sample . | FAB subtype . | Immunophenotype* . | Cytogenetic phenotype . | Cytochemistry . | ||
|---|---|---|---|---|---|---|
| Per . | NSE . | PAS . | ||||
| 1 | M0 | CD13, CD34, CD45, HLA-DR | NL | <3% | − | − |
| 2 | M1 | CD33, CD45, HLA-DR, TdT | NL | <10% | − | − |
| 3 | M1 | CD5, CD7, CD11b, CD13, HLA-DR, CD45 | NL | 20% | − | − |
| 4 | M1 | CD13, CD33, CD45 | NL | + | − | − |
| 5 | M1 | ND | ND | <10% | − | − |
| 6 | M2 | CD13, CD15, CD34, CD45, TdT | NL | + | − | − |
| 7 | M3 | CD2, CD13, CD33, CD45 | t(15:17) | + | + | − |
| 8 | M4 | CD33, CD45 | inv(16)(p32q32) | + | − | − |
| 9 | M5 | ND | ND | ND | ND | ND |
| 10 | M5a | ND | ND | + | + | − |
| 11 | M5b | CD5, CD11b, CD13, CD14, CD15, CD24, CD45 | ND | + | + | + |
| 12 | CML-B/M2 | CD2, CD13, CD33 CD34, CD38, CD45, HLA-DR | 47XY t(9:22) | + | − | − |
| 13 | CML | ND | NL | + | + | + |
Per indicates peroxidase; NSE, nonspecific esterase; PAS, periodic acid-Schiff; NL, normal (45 XX or XY); +, positive; −, negative; and ND, not done.
Positive markers listed only. All patients were negative for cytoplasmic μ, CD3, CD19, glycophorin A, and CD41.