Distribution of neutral GSLs extracted from ANLL, CML, and normal peripheral blood granulocytes5-150
| Neutral Size . | GSL bands5-150Rf . | Band . | Relative distribution of neutral GSLs (% total) per sample5-151 . | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | 12 . | 13 . | Gran . | |||
| GSL1 | 0.72 | a | 9.5 | 9.8 | 10.2 | 8.4 | 11.5 | 12.8 | 7.7 | 45.5 | 15.3 | 27.9 | 19.3 | 0.8 | 3.8 | 1.2 |
| GSL2 | 0.57 | b | 51.4 | 54.5 | 42.8 | 56.8 | 58.2 | 55.4 | 48.5 | 28.9 | 56.1 | 42.5 | 42.4 | 8.6 | 80.6 | 79.8 |
| Ratio | ||||||||||||||||
| GSL2/GSL1 | 5.4 | 5.6 | 4.2 | 6.8 | 5.1 | 4.3 | 6.3 | 0.6 | 3.7 | 1.5 | 2.2 | 10.7 | 21.2 | 66.5 | ||
| GSL3 | 0.45 | c, d | 4.2 | 6.1 | 7.6 | 14.0 | 4.3 | 4.8 | 16.9 | 5.1 | 12.2 | 4.8 | 4.3 | 8.0 | 2.7 | 0.9 |
| GSL4 | e, f | 29.4 | 25.2 | 34.5 | 12.8 | 23.6 | 21.6 | 24.1 | 17.5 | 9.3 | 22.1 | 33.9 | 65.2 | 11.2 | 15.2 | |
| 0.27 | e | 17.0 | 10.7 | 17.6 | 4.4 | 3.6 | 7.8 | 10.3 | <1.0 | 2.0 | 12.5 | 17.7 | 3.6 | 1.2 | 1.2 | |
| % GSL45-152 | 56.5 | 42.4 | 51.0 | 34.4 | 15.4 | 36.2 | 42.7 | 4.4 | 21.5 | 56.6 | 44.5 | 5.1 | 7.9 | 8.5 | ||
| 0.23 | f | 13.1 | 14.5 | 16.9 | 8.4 | 20.0 | 13.8 | 13.8 | 16.7 | 7.3 | 9.6 | 18.4 | 61.9 | 10.2 | 14.0 | |
| % GSL45-152 | 43.5 | 57.3 | 49.0 | 65.6 | 84.6 | 63.8 | 57.3 | 95.6 | 78.5 | 43.4 | 55.5 | 94.9 | 92.1 | 91.5 | ||
| Total GSLs, μg/108cells | 8.8 | 19.4 | 9.4 | 7.2 | 8.8 | 14.9 | 10.1 | 8.2 | 7.7 | 6.8 | 6.2 | 6.3 | 128.1 | 155.0 | ||
| Neutral Size . | GSL bands5-150Rf . | Band . | Relative distribution of neutral GSLs (% total) per sample5-151 . | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 9 . | 10 . | 11 . | 12 . | 13 . | Gran . | |||
| GSL1 | 0.72 | a | 9.5 | 9.8 | 10.2 | 8.4 | 11.5 | 12.8 | 7.7 | 45.5 | 15.3 | 27.9 | 19.3 | 0.8 | 3.8 | 1.2 |
| GSL2 | 0.57 | b | 51.4 | 54.5 | 42.8 | 56.8 | 58.2 | 55.4 | 48.5 | 28.9 | 56.1 | 42.5 | 42.4 | 8.6 | 80.6 | 79.8 |
| Ratio | ||||||||||||||||
| GSL2/GSL1 | 5.4 | 5.6 | 4.2 | 6.8 | 5.1 | 4.3 | 6.3 | 0.6 | 3.7 | 1.5 | 2.2 | 10.7 | 21.2 | 66.5 | ||
| GSL3 | 0.45 | c, d | 4.2 | 6.1 | 7.6 | 14.0 | 4.3 | 4.8 | 16.9 | 5.1 | 12.2 | 4.8 | 4.3 | 8.0 | 2.7 | 0.9 |
| GSL4 | e, f | 29.4 | 25.2 | 34.5 | 12.8 | 23.6 | 21.6 | 24.1 | 17.5 | 9.3 | 22.1 | 33.9 | 65.2 | 11.2 | 15.2 | |
| 0.27 | e | 17.0 | 10.7 | 17.6 | 4.4 | 3.6 | 7.8 | 10.3 | <1.0 | 2.0 | 12.5 | 17.7 | 3.6 | 1.2 | 1.2 | |
| % GSL45-152 | 56.5 | 42.4 | 51.0 | 34.4 | 15.4 | 36.2 | 42.7 | 4.4 | 21.5 | 56.6 | 44.5 | 5.1 | 7.9 | 8.5 | ||
| 0.23 | f | 13.1 | 14.5 | 16.9 | 8.4 | 20.0 | 13.8 | 13.8 | 16.7 | 7.3 | 9.6 | 18.4 | 61.9 | 10.2 | 14.0 | |
| % GSL45-152 | 43.5 | 57.3 | 49.0 | 65.6 | 84.6 | 63.8 | 57.3 | 95.6 | 78.5 | 43.4 | 55.5 | 94.9 | 92.1 | 91.5 | ||
| Total GSLs, μg/108cells | 8.8 | 19.4 | 9.4 | 7.2 | 8.8 | 14.9 | 10.1 | 8.2 | 7.7 | 6.8 | 6.2 | 6.3 | 128.1 | 155.0 | ||
Solvent was C/M/W 65:30:5 (vol/vol). Results reported are the mean of 7 independent sample determinations; SD less than 3.0%. GSL1 indicates monoglycosylceramide; GSL2, diglycosylceramide; GSL3, triglycosylceramide; and GSL4, tetraglyosylceramide.
GSLs were identified by the size of the oligosaccharide moiety, relative mobility (Rf), and immunostaining characteristics (Figure 2).
Percent distribution of major neutral GSL species as determined by scanning densitometry of DPA-stained HPTLC plates.
The percentage total GSL4 as band e (Figure 2A, Rf 0.27) or band f (doublet, Rf 0.23).