Mean fluorescence intensity units ± SEMs of CD80, CD86, and HLA-DR expression of DCs before and after stimulation with LPS or CD40L
| . | No stimulus . | LPS (P) . | CD40L (P) . |
|---|---|---|---|
| Isotype control | |||
| Uninfected | 7 ± 1 | 3 ± 1 | 7 ± 1 |
| Adβ-gal | 8 ± 2 | 3 ± 1 | 7 ± 1 |
| AdNIKdn | 8 ± 1 | 3 ± 1 | 7 ± 1 |
| AdIKK2dn | 8 ± 1 | 3 ± 1 | 7 ± 2 |
| AdIκBα | 7 ± 2 | 4 ± 0 | 7 ± 2 |
| CD80 | |||
| Uninfected | 22 ± 6 | 58 ± 2 | 43 ± 6 |
| Adβ-gal | 22 ± 5 | 56 ± 9 | 43 ± 6 |
| AdNIKdn | 20 ± 5 | 61 ± 2 | 41 ± 6 |
| AdIKK2dn | 17 ± 2 | 54 ± 9 | 18 ± 5 (< .05) |
| AdIκBα | 17 ± 4 | 22 ± 2 (< .01) | 16 ± 3 (< .05) |
| CD86 | |||
| Uninfected | 20 ± 4 | 51 ± 7 | 46 ± 5 |
| Adβ-gal | 21 ± 5 | 55 ± 3 | 46 ± 4 |
| AdNIKdn | 24 ± 5 | 58 ± 2 | 48 ± 4 |
| AdIKK2dn | 22 ± 4 | 54 ± 2 | 27 ± 5 (< .05) |
| AdIκBα | 22 ± 5 | 19 ± 5 (< .01) | 23 ± 3 (< .01) |
| HLA-DR | |||
| Uninfected | 82 ± 7 | 113 ± 10 | 137 ± 36 |
| Adβ-gal | 83 ± 3 | 117 ± 1 | 149 ± 33 |
| AdNIKdn | 78 ± 9 | 127 ± 1 | 132 ± 25 |
| AdIKK2dn | 90 ± 3 | 129 ± 6 | 79 ± 14 (< .05) |
| AdIκBα | 80 ± 8 | 69 ± 9 (< .01) | 66 ± 8 (< .05) |
| . | No stimulus . | LPS (P) . | CD40L (P) . |
|---|---|---|---|
| Isotype control | |||
| Uninfected | 7 ± 1 | 3 ± 1 | 7 ± 1 |
| Adβ-gal | 8 ± 2 | 3 ± 1 | 7 ± 1 |
| AdNIKdn | 8 ± 1 | 3 ± 1 | 7 ± 1 |
| AdIKK2dn | 8 ± 1 | 3 ± 1 | 7 ± 2 |
| AdIκBα | 7 ± 2 | 4 ± 0 | 7 ± 2 |
| CD80 | |||
| Uninfected | 22 ± 6 | 58 ± 2 | 43 ± 6 |
| Adβ-gal | 22 ± 5 | 56 ± 9 | 43 ± 6 |
| AdNIKdn | 20 ± 5 | 61 ± 2 | 41 ± 6 |
| AdIKK2dn | 17 ± 2 | 54 ± 9 | 18 ± 5 (< .05) |
| AdIκBα | 17 ± 4 | 22 ± 2 (< .01) | 16 ± 3 (< .05) |
| CD86 | |||
| Uninfected | 20 ± 4 | 51 ± 7 | 46 ± 5 |
| Adβ-gal | 21 ± 5 | 55 ± 3 | 46 ± 4 |
| AdNIKdn | 24 ± 5 | 58 ± 2 | 48 ± 4 |
| AdIKK2dn | 22 ± 4 | 54 ± 2 | 27 ± 5 (< .05) |
| AdIκBα | 22 ± 5 | 19 ± 5 (< .01) | 23 ± 3 (< .01) |
| HLA-DR | |||
| Uninfected | 82 ± 7 | 113 ± 10 | 137 ± 36 |
| Adβ-gal | 83 ± 3 | 117 ± 1 | 149 ± 33 |
| AdNIKdn | 78 ± 9 | 127 ± 1 | 132 ± 25 |
| AdIKK2dn | 90 ± 3 | 129 ± 6 | 79 ± 14 (< .05) |
| AdIκBα | 80 ± 8 | 69 ± 9 (< .01) | 66 ± 8 (< .05) |
A Student 2-sided t test for parametric data was used for statistical analysis. The null hypothesis was that expression of the respective surface molecule in adenovirus-infected cells was not different from the control uninfected cells.