MAb 3E7 enhances RTX-mediated killing of DB cells in NHS
| Condition . | 1 h . | 48 h . | ||||
|---|---|---|---|---|---|---|
| Live cells, no. . | Live cells, %* . | Dead cells, no. . | Live cells, no. . | Live cells, %* . | Dead cells, no. . | |
| NHS | 8700 ± 660 | 96 ± 0.2 | 190 ± 20 | 21 000 ± 900 | 94 ± 1 | 500 ± 30 |
| NHS/RTX | 8800 ± 250 | 72 ± 3 | 750 ± 200 | 7200 ± 1000 | 52 ± 5 | 5400 ± 700 |
| NHS/RTX/mAb 3E7 | 1300 ± 180 | 36 ± 1 | 590 ± 140 | 700 ± 350 | 6 ± 3 | 8800 ± 600 |
| Medium/RTX | 5900 ± 400 | 88 ± 4 | 260 ± 30 | 22 500 ± 1200 | 86 ± 1 | 730 ± 80 |
| Medium/RTX/mAb 3E7 | 6600 ± 470 | 91 ± 3 | 220 ± 20 | 21 500 ± 20 | 87 ± 0.1 | 650 ± 10 |
| Condition . | 1 h . | 48 h . | ||||
|---|---|---|---|---|---|---|
| Live cells, no. . | Live cells, %* . | Dead cells, no. . | Live cells, no. . | Live cells, %* . | Dead cells, no. . | |
| NHS | 8700 ± 660 | 96 ± 0.2 | 190 ± 20 | 21 000 ± 900 | 94 ± 1 | 500 ± 30 |
| NHS/RTX | 8800 ± 250 | 72 ± 3 | 750 ± 200 | 7200 ± 1000 | 52 ± 5 | 5400 ± 700 |
| NHS/RTX/mAb 3E7 | 1300 ± 180 | 36 ± 1 | 590 ± 140 | 700 ± 350 | 6 ± 3 | 8800 ± 600 |
| Medium/RTX | 5900 ± 400 | 88 ± 4 | 260 ± 30 | 22 500 ± 1200 | 86 ± 1 | 730 ± 80 |
| Medium/RTX/mAb 3E7 | 6600 ± 470 | 91 ± 3 | 220 ± 20 | 21 500 ± 20 | 87 ± 0.1 | 650 ± 10 |
Values shown are averages ± SDs based on duplicate determinations with 3 different NHSs and are representative of 4 similar experiments. The NHS/RTX/mAb 3E7 sample had fewer live cells than NHS/RTX at both 1 hour and 48 hours (P < 10−3). More dead cells were present in the NHS/RTX/mAb 3E7 sample than in NHS/RTX at 48 hours (P < 10−3).
Percentage of live cells was calculated for each individual determination and then averaged.