Capacity of the adherent cells to generate nonadherent CD34− cells
| . | Number of NA cells generated per 5000 adherent cells . | ||
|---|---|---|---|
| Week 1 . | Week 2 . | Week 3 . | |
| Unstimulated adherent cells | 180 ± 74 | 100 ± 68 | 40 ± 18 |
| Adherent cells stimulated with SCF | 396 ± 118 | 270 ± 91 | 100 ± 35 |
| . | Number of NA cells generated per 5000 adherent cells . | ||
|---|---|---|---|
| Week 1 . | Week 2 . | Week 3 . | |
| Unstimulated adherent cells | 180 ± 74 | 100 ± 68 | 40 ± 18 |
| Adherent cells stimulated with SCF | 396 ± 118 | 270 ± 91 | 100 ± 35 |
Six-week-old adherent cells were transferred to 96-well microtiter plates (5000 cells per well) in triplicate and were cultured for 3 additional weeks in serum-free medium in the presence or absence of 100 ng/mL SCF. The number of NA cells generated each week was recorded. Data represent the means ± standard deviations of 3 experiments.