Quantification of RBC membrane proteins in the band 3(−/−) mouse by immunoblotting
| Protein . | Antibody . | Band 3(−/−) mouse, % of control* . |
|---|---|---|
| Protein 4.2 | Polyclonal | 0, 0 |
| RhAG | Polyclonal | 9, 6 |
| Rh polypeptides | Polyclonal | 1, 0.06 |
| CD47 | C-terminal polyclonal | 84, 83 |
| Glycophorin C | Polyclonal | 100, 100 |
| Protein 4.1 | Polyclonal | 100 (± 2) |
| Aquaporin, total | Polyclonal | 69, 79 |
| Crry | Polyclonal | 143, 139 |
| Protein . | Antibody . | Band 3(−/−) mouse, % of control* . |
|---|---|---|
| Protein 4.2 | Polyclonal | 0, 0 |
| RhAG | Polyclonal | 9, 6 |
| Rh polypeptides | Polyclonal | 1, 0.06 |
| CD47 | C-terminal polyclonal | 84, 83 |
| Glycophorin C | Polyclonal | 100, 100 |
| Protein 4.1 | Polyclonal | 100 (± 2) |
| Aquaporin, total | Polyclonal | 69, 79 |
| Crry | Polyclonal | 143, 139 |
Values obtained from the RBCs of 2 different band 3(−/−) animals. Amounts of protein are expressed as the percentage of control after normalization to the amount of protein 4.1 in the 2 membrane preparations.