Time-course changes in surface coverage in perfusion of blood from type 2A and 2B VWD patients and from controls under a high shear rate (1500 s−1)
| Patient . | 1-minute perfusion, % . | 7-minute perfusion, % . |
|---|---|---|
| 2A-1 | 0.9 ± 0.3 | 10.2 ± 2.4* |
| -2 | 0.2 ± 0.05 | 7.6 ± 3.0* |
| -3 | 0.1 ± 0.05 | 2.6 ± 0.3* |
| 2B-1 | 0.2 ± 0.15 | 8.5 ± 3.2* |
| -2 | 0.1 ± 0.05 | 12.7 ± 1.5* |
| -3 | 0.5 ± 0.1 | 38.2 ± 5.6 |
| -4 | 0.7 ± 0.2 | 43.6 ± 5.5 |
| Control-1 | 0.6 ± 0.25 | 37.3 ± 6.6 |
| -2 | 1.1 ± 0.3 | 42.4 ± 8.5 |
| -3 | 0.8 ± 0.2 | 48.3 ± 8.2 |
| -4 | 0.5 ± 0.15 | 40.1 ± 2.2 |
| -5 | 1.5 ± 0.4 | 42.7 ± 3.4 |
| Patient . | 1-minute perfusion, % . | 7-minute perfusion, % . |
|---|---|---|
| 2A-1 | 0.9 ± 0.3 | 10.2 ± 2.4* |
| -2 | 0.2 ± 0.05 | 7.6 ± 3.0* |
| -3 | 0.1 ± 0.05 | 2.6 ± 0.3* |
| 2B-1 | 0.2 ± 0.15 | 8.5 ± 3.2* |
| -2 | 0.1 ± 0.05 | 12.7 ± 1.5* |
| -3 | 0.5 ± 0.1 | 38.2 ± 5.6 |
| -4 | 0.7 ± 0.2 | 43.6 ± 5.5 |
| Control-1 | 0.6 ± 0.25 | 37.3 ± 6.6 |
| -2 | 1.1 ± 0.3 | 42.4 ± 8.5 |
| -3 | 0.8 ± 0.2 | 48.3 ± 8.2 |
| -4 | 0.5 ± 0.15 | 40.1 ± 2.2 |
| -5 | 1.5 ± 0.4 | 42.7 ± 3.4 |
Whole blood containing mepacrine-labeled platelets was perfused through a collagen-coated glass surface under a shear rate of 1500 s−1. Surface coverage by thrombi within a defined area (211 × 317 μm each) was evaluated based on epifluorescence images taken at 1 and 7 minutes after the beginning of platelet-surface interaction during perfusion. Data represent the mean ± SD of 5 areas randomly selected in each single perfusion.
Statistically significant differences from controls (P< .01) by 1-way factorial analysis of variance (ANOVA) and the Scheffé method as described in the legend to Figure 4.