Expression of human lineage markers in the bone marrow, spleen, and peripheral blood of NOD/SCID and NOD/SCID/MPSVII mice at 6 and 12 weeks after transplantation of cord blood CD34+cells or mobilized peripheral blood CD34+ cells
| . | CD45+ . | % of Human CD45+ cells . | ||||
|---|---|---|---|---|---|---|
| CD19+ . | CD14+/CD66b− . | CD66b+/CD14− . | CD66b+/CD14+ . | Undetermined . | ||
| Cord blood CD34+cells | ||||||
| NOD/SCID/MPSVII | ||||||
| 6 wk | ||||||
| BM | 57.9 ± 20.4 | 54.8 ± 6.9 | 4.8 ± 1.1 | 12.9 ± 8.5 | 0.4 ± 0.2 | 27.3 ± 5.9 |
| SPL | 18.8 ± 14.5 | 82.5 ± 5.0 | 1.9 ± 1.0 | 0.6 ± 0.4 | 0 | 15.0 ± 4.9 |
| PB | 10.4 ± 7.5 | 65.5 ± 6.8 | 7.0 ± 4.2 | 1.4 ± 0.4 | 0 | 26.1 ± 3.3 |
| 12 wk | ||||||
| BM | 60.3 ± 28.9 | 42.3 ± 15.5* | 6.4 ± 1.6 | 29.8 ± 19.5† | 1.2 ± 0.7‡ | 20.5 ± 4.4 |
| SPL | 29.5 ± 18.7 | 79.6 ± 4.0 | 1.5 ± 0.9 | 0.6 ± 0.4 | 0 | 18.1 ± 3.4 |
| PB | 10.1 ± 8.0 | 58.5 ± 4.0 | 8.4 ± 3.1 | 1.5 ± 1.1 | 0 | 31.6 ± 4.0 |
| NOD/SCID | ||||||
| 12 wk | ||||||
| BM | 34.2 ± 33.6 | 59.7 ± 11.7* | 7.0 ± 5.0 | 9.1 ± 9.0† | 0.3 ± 0.4‡ | 24.0 ± 6.8 |
| SPL | 26.0 ± 28.2 | 83.9 ± 7.2 | 1.3 ± 0.7 | 0.4 ± 0.5 | 0 | 14.5 ± 7.0 |
| PB | 9.0 ± 13.3 | 61.9 ± 11.3 | 6.1 ± 2.7 | 0.9 ± 0.8 | 0 | 31.0 ± 11.0 |
| Mobilized peripheral blood CD34+cells | ||||||
| NOD/SCID/MPSVII | ||||||
| 12 wk | ||||||
| BM | 16.3 ± 17.5 | 52.3 ± 13.6 | 9.6 ± 5.9 | 4.8 ± 6.9 | 0.1 ± 0.3 | 33.3 ± 6.7 |
| SPL | ND | ND | ND | ND | ND | ND |
| PB | 1.4 ± 1.1 | 56.5 ± 8.5 | 10.5 ± 3.7 | 0.8 ± 1.0 | 0 | 32.3 ± 5.1 |
| NOD/SCID | ||||||
| 12 wk | ||||||
| BM | 14.9 ± 25.6 | 55.6 ± 11.8 | 6.4 ± 3.6 | 3.1 ± 4.3 | 0.2 ± 0.2 | 30.8 ± 11.2 |
| SPL | ND | ND | ND | ND | ND | ND |
| PB | 2.6 ± 4.9 | 57.3 ± 6.3 | 9.7 ± 6.5 | 0.3 ± 0.5 | 0 | 32.7 ± 5.7 |
| . | CD45+ . | % of Human CD45+ cells . | ||||
|---|---|---|---|---|---|---|
| CD19+ . | CD14+/CD66b− . | CD66b+/CD14− . | CD66b+/CD14+ . | Undetermined . | ||
| Cord blood CD34+cells | ||||||
| NOD/SCID/MPSVII | ||||||
| 6 wk | ||||||
| BM | 57.9 ± 20.4 | 54.8 ± 6.9 | 4.8 ± 1.1 | 12.9 ± 8.5 | 0.4 ± 0.2 | 27.3 ± 5.9 |
| SPL | 18.8 ± 14.5 | 82.5 ± 5.0 | 1.9 ± 1.0 | 0.6 ± 0.4 | 0 | 15.0 ± 4.9 |
| PB | 10.4 ± 7.5 | 65.5 ± 6.8 | 7.0 ± 4.2 | 1.4 ± 0.4 | 0 | 26.1 ± 3.3 |
| 12 wk | ||||||
| BM | 60.3 ± 28.9 | 42.3 ± 15.5* | 6.4 ± 1.6 | 29.8 ± 19.5† | 1.2 ± 0.7‡ | 20.5 ± 4.4 |
| SPL | 29.5 ± 18.7 | 79.6 ± 4.0 | 1.5 ± 0.9 | 0.6 ± 0.4 | 0 | 18.1 ± 3.4 |
| PB | 10.1 ± 8.0 | 58.5 ± 4.0 | 8.4 ± 3.1 | 1.5 ± 1.1 | 0 | 31.6 ± 4.0 |
| NOD/SCID | ||||||
| 12 wk | ||||||
| BM | 34.2 ± 33.6 | 59.7 ± 11.7* | 7.0 ± 5.0 | 9.1 ± 9.0† | 0.3 ± 0.4‡ | 24.0 ± 6.8 |
| SPL | 26.0 ± 28.2 | 83.9 ± 7.2 | 1.3 ± 0.7 | 0.4 ± 0.5 | 0 | 14.5 ± 7.0 |
| PB | 9.0 ± 13.3 | 61.9 ± 11.3 | 6.1 ± 2.7 | 0.9 ± 0.8 | 0 | 31.0 ± 11.0 |
| Mobilized peripheral blood CD34+cells | ||||||
| NOD/SCID/MPSVII | ||||||
| 12 wk | ||||||
| BM | 16.3 ± 17.5 | 52.3 ± 13.6 | 9.6 ± 5.9 | 4.8 ± 6.9 | 0.1 ± 0.3 | 33.3 ± 6.7 |
| SPL | ND | ND | ND | ND | ND | ND |
| PB | 1.4 ± 1.1 | 56.5 ± 8.5 | 10.5 ± 3.7 | 0.8 ± 1.0 | 0 | 32.3 ± 5.1 |
| NOD/SCID | ||||||
| 12 wk | ||||||
| BM | 14.9 ± 25.6 | 55.6 ± 11.8 | 6.4 ± 3.6 | 3.1 ± 4.3 | 0.2 ± 0.2 | 30.8 ± 11.2 |
| SPL | ND | ND | ND | ND | ND | ND |
| PB | 2.6 ± 4.9 | 57.3 ± 6.3 | 9.7 ± 6.5 | 0.3 ± 0.5 | 0 | 32.7 ± 5.7 |
ND indicates not determined.
Statistically significant difference between the CD19+ population in the bone marrow of NOD/SCID and NOD/SCID/MPSVII mice at 12 weeks after transplantation (P = .049).
Statistically significant difference between the CD66b+/CD14− population in the bone marrow of NOD/SCID and NOD/SCID/MPSVII mice at 12 weeks after transplantation (P = .022).
Statistically significant difference between the CD66b+/CD14+ population in the bone marrow of NOD/SCID and NOD/SCID/MPSVII at 12 weeks after transplantation (P = .015).