Summary of oligonucleotides for gel shifts and mutagenesis
| Oligonucleotide . | Sequence . |
|---|---|
| FPA (−3994 to −3967) | GCTGGAACCCGGGGCGGGCGGGCTCAGG |
| FPA-GC-g mt | GCTGGAACCCGGGGCtGtCGGGCTCAGG |
| FPB (−3957 to −3933) | AGGCGGGGGAGGGGGCGGCGACCCG |
| FPB-GC-f mt | AGGCGGGGGAtGGttCGGCGACCCG |
| FPC (−3876 to −3845) | CATGCCGGGGTCCCGGGGAGGGCGGGGGTCGG |
| FPD (−3816 to −3787) | GGGTGGGGAAGGGGTGGGGGGGAGGGGCCC |
| FPD-GT-d mt | GGGTGGGGAAGGGtTGGGGGGGAGGGGCCC |
| −3766/−3725 | GGGGCTGGGCGGGCGGGTGCTCTGCCACGAGACATTGGCCG |
| USF-1 mt | GGGGCTGGGCGGGCGGGTGCTCTGCCAtcAGACATTGGCCG |
| −3724/−3682 | CGCCGGCCTCCGCCCCCGCCTCTCCGCCAATCACCGCCCGCCT |
| NF-Y mt | CGCCGGCCTCCGCCCCCGCCTCTCCGCtAtTCACCGCCCGCCT |
| Oligonucleotide . | Sequence . |
|---|---|
| FPA (−3994 to −3967) | GCTGGAACCCGGGGCGGGCGGGCTCAGG |
| FPA-GC-g mt | GCTGGAACCCGGGGCtGtCGGGCTCAGG |
| FPB (−3957 to −3933) | AGGCGGGGGAGGGGGCGGCGACCCG |
| FPB-GC-f mt | AGGCGGGGGAtGGttCGGCGACCCG |
| FPC (−3876 to −3845) | CATGCCGGGGTCCCGGGGAGGGCGGGGGTCGG |
| FPD (−3816 to −3787) | GGGTGGGGAAGGGGTGGGGGGGAGGGGCCC |
| FPD-GT-d mt | GGGTGGGGAAGGGtTGGGGGGGAGGGGCCC |
| −3766/−3725 | GGGGCTGGGCGGGCGGGTGCTCTGCCACGAGACATTGGCCG |
| USF-1 mt | GGGGCTGGGCGGGCGGGTGCTCTGCCAtcAGACATTGGCCG |
| −3724/−3682 | CGCCGGCCTCCGCCCCCGCCTCTCCGCCAATCACCGCCCGCCT |
| NF-Y mt | CGCCGGCCTCCGCCCCCGCCTCTCCGCtAtTCACCGCCCGCCT |
DNA sequences for wild-type double-stranded oligonucleotides were used as probes or competitive oligonucleotides in gel shift assays. Single-stranded mutant oligonucleotides were used in the preparation of mutant reporter gene constructs for transient transfection experiments. The consensus sequences of the GC/GT-box, E-box, and CAAT-box elements are underlined. Lowercase letters indicate the bases mutated.