Top 50 human platelet-expressed genes
| Accession no. . | Gene symbol . | AD values, range* . | Gene transcript† . | Leukocyte expression‡ . |
|---|---|---|---|---|
| M17733 | TMSB4X | 140 142-307 852 | Thymosin β4 mRNA, complete cds | + |
| X99076 | NRGN | 101 510-148 279 | Neurogranin gene | + |
| M25079 | HBB | 40 839-229 556 | β-globin mRNA, complete cds | + |
| M25915 | CLU | 84 720-140 246 | Complement cytolysis inhibitor (clusterin) complete cds | − |
| J04755 | FTHP1 | 82 980-148 621 | Ferritin H processed pseudogene, complete cds | − |
| D78361 | OAZ1 | 73 098-118 140 | mRNA for omithine decarboxylase antizyme | − |
| X04409 | GNAS | 77 761-94 781 | mRNA for coupling protein G(s) α-subunit (alpha-S1) | − |
| M25897 | PF4 | 62 811-126 908 | Platelet factor 4 mRNA, complete cds | − |
| AB021288 | B2M | 61 689-108 921 | β2-microglobulin | + |
| X00351 | ACTB | 25 143-73 775 | mRNA for β-actin | − |
| D21261 | TAGLN2 | 76 687-101 931 | mRNA for KIAA0120 gene | + |
| AL031670 | FTLL1 | 69 865-99 966 | Ferritin, light polypeptide 1 | + |
| U59632 | GPIIB | 41 404-110 328 | Platelet glycoprotein Ibβ chain mRNA | − |
| M21121 | CCL5 | 47 308-106 399 | T-cell-specific protein (RANTES) mRNA, complete cds | − |
| X13710 | GPX1 | 41 318-96 878 | Unspliced mRNA for glutathione peroxidase | − |
| J00153 | HBA1 | 21 326-144 201 | Alpha globin gene cluster on chromosome 16 | + |
| M22919 | MYL6 | 46 337-106 833 | Nonmuscle/smooth muscle alkali myosin light chain gene | + |
| L20941 | FTH1 | 52 787-74 763 | Ferritin heavy chain mRNA, complete cds | − |
| J03040 | SPARC | 51 156-74 261 | SPARC/osteonectin mRNA, complete cds | − |
| X56009 | GNAS | 45 543-72 096 | GSA mRNA for α subunit of GsGTP binding protein | − |
| X58536 | HLA | 31 183-82 613 | mRNA for major HLA class I locus C heavy chain | + |
| M54995 | PPBP | 46 571-67 169 | Connective tissue activation peptide III mRNA | − |
| U34995 | GAPD | 35 095-70 250 | Normal keratinocyte substraction library mRNA, clone H22a | + |
| L40399 | MLM3 | 32 107-73 364 | Clone zap112 (mutL protein homolog 3) mRNA | − |
| X77548 | NCOA4 | 31 452-61 036 | cDNA for RFG (RETproto-oncogene RET/PTC3) | − |
| U90551 | H2AFL | 35 086-51 892 | Histone 2A-like protein (H2A/I) mRNA | − |
| M11353 | H3F3A | 31 614-55 813 | H3.3 histone class C mRNA | − |
| Z12962 | RPL41 | 36 003-54 853 | mRNA for homologue to yeast ribosomal protein L41 | + |
| X06956 | TUBA1 | 20 988-61 798 | HALPHA 44 gene for α-tubulin | − |
| AB028950 | TLN1 | 24 571-58 611 | mRNA for KIAA 1027 protein | − |
| Y12711 | PGRMC1 | 33 680-43 174 | mRNA for putative progesterone binding protein | − |
| M16279 | MIC2 | 30 894-48 166 | Integrated membrane protein (MIC2) mRNA | − |
| D78577 | YWHAH | 24 785-50 437 | Brain 14-3-3 protein β-chain | − |
| AF070585 | TOP3B | 20 027-67 945 | Clone 24675, unknown cDNA | − |
| AA524802 | Unknown | 23 846-39 481 | CDNA, IMAGE clone 954213 | − |
| AB009010 | UBC | 28 745-38 389 | mRNA for polyubiquitin UbC | + |
| X57985 | H2AFQ | 21 678-52 108 | Genes for histones H2B.1 and H2A | − |
| X54304 | MLCB | 25 733-34 109 | mRNA for myosin regulatory light chain | − |
| M14539 | F13A1 | 23 691-48 474 | Factor XIII subunit α-polypeptide mRNA, 3′ end | − |
| AI540958 | Unknown | 24 872-41 118 | cDNA, PEC 1.2_15_HOI.r 5′ end /clon | − |
| AL050396 | FLNA | 13 634-55 235 | cDNA DKFZp 586K1720 | − |
| X56841 | HLA-E | 12 890-49 327 | Nonclassical MHC class I antigen gene | − |
| M26252 | PKM2 | 15 450-47 786 | TCB (cytosolic thyroid hormone-binding protein) | − |
| M14630 | PTMA | 19 314-45 088 | Prothymosin alpha mRNA | − |
| AF045229 | RGS10 | 19 156-34 243 | Regulator of G protein signaling 10 mRNA | − |
| AA477898 | Unknown | 16 863-44 756 | cDNA, Z&34f08.rl 5′ end | − |
| X95404 | FL1 | 15 216-37 456 | mRNA for nonmuscle type cofilin | − |
| M34480 | ITGA2B | 8 627-45 495 | Platelet glycoprotein IIb (GPIIb) mRNA | − |
| Z83738 | H2BFE | 18 001-31 306 | HH2B/e gene | − |
| L19779 | H2AFO | 17 319-38 951 | Histone H2A.2 mRNA, complete cds | − |
| Accession no. . | Gene symbol . | AD values, range* . | Gene transcript† . | Leukocyte expression‡ . |
|---|---|---|---|---|
| M17733 | TMSB4X | 140 142-307 852 | Thymosin β4 mRNA, complete cds | + |
| X99076 | NRGN | 101 510-148 279 | Neurogranin gene | + |
| M25079 | HBB | 40 839-229 556 | β-globin mRNA, complete cds | + |
| M25915 | CLU | 84 720-140 246 | Complement cytolysis inhibitor (clusterin) complete cds | − |
| J04755 | FTHP1 | 82 980-148 621 | Ferritin H processed pseudogene, complete cds | − |
| D78361 | OAZ1 | 73 098-118 140 | mRNA for omithine decarboxylase antizyme | − |
| X04409 | GNAS | 77 761-94 781 | mRNA for coupling protein G(s) α-subunit (alpha-S1) | − |
| M25897 | PF4 | 62 811-126 908 | Platelet factor 4 mRNA, complete cds | − |
| AB021288 | B2M | 61 689-108 921 | β2-microglobulin | + |
| X00351 | ACTB | 25 143-73 775 | mRNA for β-actin | − |
| D21261 | TAGLN2 | 76 687-101 931 | mRNA for KIAA0120 gene | + |
| AL031670 | FTLL1 | 69 865-99 966 | Ferritin, light polypeptide 1 | + |
| U59632 | GPIIB | 41 404-110 328 | Platelet glycoprotein Ibβ chain mRNA | − |
| M21121 | CCL5 | 47 308-106 399 | T-cell-specific protein (RANTES) mRNA, complete cds | − |
| X13710 | GPX1 | 41 318-96 878 | Unspliced mRNA for glutathione peroxidase | − |
| J00153 | HBA1 | 21 326-144 201 | Alpha globin gene cluster on chromosome 16 | + |
| M22919 | MYL6 | 46 337-106 833 | Nonmuscle/smooth muscle alkali myosin light chain gene | + |
| L20941 | FTH1 | 52 787-74 763 | Ferritin heavy chain mRNA, complete cds | − |
| J03040 | SPARC | 51 156-74 261 | SPARC/osteonectin mRNA, complete cds | − |
| X56009 | GNAS | 45 543-72 096 | GSA mRNA for α subunit of GsGTP binding protein | − |
| X58536 | HLA | 31 183-82 613 | mRNA for major HLA class I locus C heavy chain | + |
| M54995 | PPBP | 46 571-67 169 | Connective tissue activation peptide III mRNA | − |
| U34995 | GAPD | 35 095-70 250 | Normal keratinocyte substraction library mRNA, clone H22a | + |
| L40399 | MLM3 | 32 107-73 364 | Clone zap112 (mutL protein homolog 3) mRNA | − |
| X77548 | NCOA4 | 31 452-61 036 | cDNA for RFG (RETproto-oncogene RET/PTC3) | − |
| U90551 | H2AFL | 35 086-51 892 | Histone 2A-like protein (H2A/I) mRNA | − |
| M11353 | H3F3A | 31 614-55 813 | H3.3 histone class C mRNA | − |
| Z12962 | RPL41 | 36 003-54 853 | mRNA for homologue to yeast ribosomal protein L41 | + |
| X06956 | TUBA1 | 20 988-61 798 | HALPHA 44 gene for α-tubulin | − |
| AB028950 | TLN1 | 24 571-58 611 | mRNA for KIAA 1027 protein | − |
| Y12711 | PGRMC1 | 33 680-43 174 | mRNA for putative progesterone binding protein | − |
| M16279 | MIC2 | 30 894-48 166 | Integrated membrane protein (MIC2) mRNA | − |
| D78577 | YWHAH | 24 785-50 437 | Brain 14-3-3 protein β-chain | − |
| AF070585 | TOP3B | 20 027-67 945 | Clone 24675, unknown cDNA | − |
| AA524802 | Unknown | 23 846-39 481 | CDNA, IMAGE clone 954213 | − |
| AB009010 | UBC | 28 745-38 389 | mRNA for polyubiquitin UbC | + |
| X57985 | H2AFQ | 21 678-52 108 | Genes for histones H2B.1 and H2A | − |
| X54304 | MLCB | 25 733-34 109 | mRNA for myosin regulatory light chain | − |
| M14539 | F13A1 | 23 691-48 474 | Factor XIII subunit α-polypeptide mRNA, 3′ end | − |
| AI540958 | Unknown | 24 872-41 118 | cDNA, PEC 1.2_15_HOI.r 5′ end /clon | − |
| AL050396 | FLNA | 13 634-55 235 | cDNA DKFZp 586K1720 | − |
| X56841 | HLA-E | 12 890-49 327 | Nonclassical MHC class I antigen gene | − |
| M26252 | PKM2 | 15 450-47 786 | TCB (cytosolic thyroid hormone-binding protein) | − |
| M14630 | PTMA | 19 314-45 088 | Prothymosin alpha mRNA | − |
| AF045229 | RGS10 | 19 156-34 243 | Regulator of G protein signaling 10 mRNA | − |
| AA477898 | Unknown | 16 863-44 756 | cDNA, Z&34f08.rl 5′ end | − |
| X95404 | FL1 | 15 216-37 456 | mRNA for nonmuscle type cofilin | − |
| M34480 | ITGA2B | 8 627-45 495 | Platelet glycoprotein IIb (GPIIb) mRNA | − |
| Z83738 | H2BFE | 18 001-31 306 | HH2B/e gene | − |
| L19779 | H2AFO | 17 319-38 951 | Histone H2A.2 mRNA, complete cds | − |
Gene expression quantifications were calculated as the average difference (AD) value (matched versus mismatched oligonucleotides) for each probe set using Affymetrix GeneChip software, version 4.01. The range of values from 3 distinct platelet microarrays is shown; the normalization value for all microarray analyses was 250.
Transcripts are rank-ordered (highest to lowest) using BRB-ArrayTools software by log-intensities of AD values obtained from 3 different healthy donors; 33 of the top 40 transcripts were listed among the top 50 in all 3 microarray sets.
Leukocyte expression was determined by microarray analysis using purified peripheral blood leukocytes, followed by construction of rank-intensity plots for comparison to platelet top 50 transcripts.20 Top leukocyte-derived transcripts identified within the ranked top 50 platelet transcripts are depicted by a (+) present, or (−) absent.
cds indicates coding sequence.