Delayed PMN apoptosis inCD18−/−/IL6−/−mice
| . | Apoptotic cells in percent of total cell number . | |
|---|---|---|
| CD16 expression . | DNA content . | |
| CD18+/+/IL-6+/+, n = 6 | ||
| 0 h | 26.6 ± 11.6 | 3.1 ± 1.0 |
| 4 h | 68.7 ± 5.8 | 19.7 ± 5.1 |
| CD18−/−/IL-6−/−, n = 5 | ||
| 0 h | 6.6 ± 3.9* | 0.6 ± 0.3† |
| 4 h | 19.1 ± 5.4† | 9.9 ± 2.3† |
| . | Apoptotic cells in percent of total cell number . | |
|---|---|---|
| CD16 expression . | DNA content . | |
| CD18+/+/IL-6+/+, n = 6 | ||
| 0 h | 26.6 ± 11.6 | 3.1 ± 1.0 |
| 4 h | 68.7 ± 5.8 | 19.7 ± 5.1 |
| CD18−/−/IL-6−/−, n = 5 | ||
| 0 h | 6.6 ± 3.9* | 0.6 ± 0.3† |
| 4 h | 19.1 ± 5.4† | 9.9 ± 2.3† |
Flow cytometric analysis of CD16 expression on the cell surface of PMNs measured in whole blood samples using the PE-labeled anti-CD16 mAb and flow cytometric analysis of DNA content of isolated PI-stained PMNs at 0 and 4 hours after the onset of culture. Data represent mean ± SD.
P < .01.
P < .001.