How cell type may determine the risk of insertional mutagenesis
| Level . | Before insertion . | After insertion . |
|---|---|---|
| Genome | Number of pre-existing mutations | Potential for secondary mutations |
| Number and size of activated, potentially disruptible “dangerous” loci susceptible to transgene insertion | Proliferation capacity/propensity | |
| Cell-transgene interactions, direct | Host factors participating in preintegration complex | Recognition of transgene regulatory elements and expression level of transgene |
| Cell-cycle status | Type and number of mechanisms supporting transgene side effect | |
| Cellular response, indirect | Response balance to “danger” signals | |
| Potential for escape from senescence | ||
| Number of generations following genetic modification | ||
| Level of differentiation |
| Level . | Before insertion . | After insertion . |
|---|---|---|
| Genome | Number of pre-existing mutations | Potential for secondary mutations |
| Number and size of activated, potentially disruptible “dangerous” loci susceptible to transgene insertion | Proliferation capacity/propensity | |
| Cell-transgene interactions, direct | Host factors participating in preintegration complex | Recognition of transgene regulatory elements and expression level of transgene |
| Cell-cycle status | Type and number of mechanisms supporting transgene side effect | |
| Cellular response, indirect | Response balance to “danger” signals | |
| Potential for escape from senescence | ||
| Number of generations following genetic modification | ||
| Level of differentiation |