Comparison of boiled and untreated ubiquitin on LPS-stimulated TNF-α secretion of whole blood and PBMNCs
| Ubiquitin, ng/mL . | WB TNF-α production, % control . | PBMNC TNF-α production, % control . | ||
|---|---|---|---|---|
| Boiled . | Native . | Boiled . | Native . | |
| 0 | 100 | 100 | 100 | 100 |
| 500 | 66 ± 5.9 | 59 ± 4.6 | 61 ± 4.6 | 62 ± 3.6 |
| 1000 | 55 ± 4.1 | 57 ± 7.4 | 56 ± 4.1 | 57 ± 4.5 |
| Ubiquitin, ng/mL . | WB TNF-α production, % control . | PBMNC TNF-α production, % control . | ||
|---|---|---|---|---|
| Boiled . | Native . | Boiled . | Native . | |
| 0 | 100 | 100 | 100 | 100 |
| 500 | 66 ± 5.9 | 59 ± 4.6 | 61 ± 4.6 | 62 ± 3.6 |
| 1000 | 55 ± 4.1 | 57 ± 7.4 | 56 ± 4.1 | 57 ± 4.5 |
Whole blood (WB) and PBMNC cultures (n = 4) from healthy donors were incubated for 4 hours with 0, 500, and 1000 ng/mL exogenous boiled or native ubiquitin in the presence of LPS (100 ng/mL). Percent control is percent TNF-α secretion of the cultures incubated without exogenous ubiquitin (= 100%). Data represent mean ± SEM.