Table 2. Effect of exogenous ubiquitin... | American Society of Hematology

Table 2.

Effect of exogenous ubiquitin on zymosan- and S aureus-induced TNF-α production of human PBMNCs and whole blood

Ubiquitin, ng/mL	Zymosan-induced TNF-α production		S aureus-induced TNF-α production
Ubiquitin, ng/mL	WB, pg/mL	PBMNC, pg/10⁶PBMNC	WB, pg/mL	PBMNC, pg/10⁶ PBMNC
0	2960 ± 446	2516 ± 810	1474 ± 366	4872 ± 1080
100	2798 ± 535	2304 ± 630	1359 ± 295	5018 ± 1108
500	2466 ± 443	2560 ± 750	1467 ± 406	5118 ± 992
1000	2676 ± 592	2340 ± 648	1549 ± 277	4972 ± 808

Ubiquitin, ng/mL	Zymosan-induced TNF-α production		S aureus-induced TNF-α production
Ubiquitin, ng/mL	WB, pg/mL	PBMNC, pg/10⁶PBMNC	WB, pg/mL	PBMNC, pg/10⁶ PBMNC
0	2960 ± 446	2516 ± 810	1474 ± 366	4872 ± 1080
100	2798 ± 535	2304 ± 630	1359 ± 295	5018 ± 1108
500	2466 ± 443	2560 ± 750	1467 ± 406	5118 ± 992
1000	2676 ± 592	2340 ± 648	1549 ± 277	4972 ± 808

Whole blood (WB) and PBMNC cultures (n = 6) from healthy donors were incubated for 4 hours with 0, 100, 500, and 1000 ng/mL exogenous ubiquitin in the presence of zymosan (100 μg/mL) or heat killed S aureus (10⁵ cells/mL). Data represent mean ± SEM.

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