Table 1. PCR primers for the... | American Society of Hematology

Table 1.

PCR primers for the amplification of ADAMTS13exons and adjacent intron sequences chosen from the published sequence (GenBank accession numbers AL158826 and AC002325)

Exon	Sense	Antisense	AT °C
7	ggg tcg acc cgg gtc gga aaa ctc gct ggc	aga gag agg tgg cgc tgg cgg ccc agc ggg	68
10 + 11	ggg act ctc tgt gtg tgt tgg g	tga acc aca gat ggt cac ttt ggc tcc	62
12	gtg cca tgt agt ctc cca gtg aca aca cc	cca gag cct gaa cca ctt tgc cca gtg c	65
15	caa ttt ttc ccg acc agc taa gat cag ctc	act tgc ggg tga tgt cag aag tga ggg	64
16	ctg tgg ctc ctt aga gga ggg ctg ggg	cgc tga atg aat aaa tga acg aat cag gg	64
20	gca gct ggg cta tac ctt ccc ctg gg	aat ggg tgc tcc tcg ttc tcc cag cc	68
21	gga tcg ctg ggt cct cag agg agg ccc	gaa cga ggc aag atg caa gtg gac agg	65
22	gag gtg tcc agt gag cct ggg ctg cag tcc	ggc atg gag cca agg ctc agg tct cc	67
26	cct cct ggt ctc ctt cct cag ctt gg	tgc cag gaa tgg ggc atg cag cgt c	67
27	ctt gaa ctc ggc tca gtc tac cct g	ttc cat ctc agt cag ttc cct ccg	62
28	acg gct ccc tgg ctg tga gtt gtc c	ggg tcc cct agc cct gca cgc cc	68
29	ccg tga gtg cta att att act tgt gg	ggg ttt tca agc cag gtc tgg aca gc	61

Exon	Sense	Antisense	AT °C
7	ggg tcg acc cgg gtc gga aaa ctc gct ggc	aga gag agg tgg cgc tgg cgg ccc agc ggg	68
10 + 11	ggg act ctc tgt gtg tgt tgg g	tga acc aca gat ggt cac ttt ggc tcc	62
12	gtg cca tgt agt ctc cca gtg aca aca cc	cca gag cct gaa cca ctt tgc cca gtg c	65
15	caa ttt ttc ccg acc agc taa gat cag ctc	act tgc ggg tga tgt cag aag tga ggg	64
16	ctg tgg ctc ctt aga gga ggg ctg ggg	cgc tga atg aat aaa tga acg aat cag gg	64
20	gca gct ggg cta tac ctt ccc ctg gg	aat ggg tgc tcc tcg ttc tcc cag cc	68
21	gga tcg ctg ggt cct cag agg agg ccc	gaa cga ggc aag atg caa gtg gac agg	65
22	gag gtg tcc agt gag cct ggg ctg cag tcc	ggc atg gag cca agg ctc agg tct cc	67
26	cct cct ggt ctc ctt cct cag ctt gg	tgc cag gaa tgg ggc atg cag cgt c	67
27	ctt gaa ctc ggc tca gtc tac cct g	ttc cat ctc agt cag ttc cct ccg	62
28	acg gct ccc tgg ctg tga gtt gtc c	ggg tcc cct agc cct gca cgc cc	68
29	ccg tga gtg cta att att act tgt gg	ggg ttt tca agc cag gtc tgg aca gc	61

Published primers were used for the remaining exons.18 Sense and antisense primers are both given in 5′-3′ direction. Screening for identified mutations among relatives of patients and 50 normal controls (100 alleles) was done either directly by polyacrylamide gel electrophoresis (PAGE) of PCR products in the case of a dinucleotide deletion, or by PAGE after restriction enzyme digest (Table 2). AT indicates annealing temperature.

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