Table 1. Oligonucleotide primers used... | American Society of Hematology

Table 1.

Oligonucleotide primers used for amplification and construction of scFvs

p5.2V_H-1

5′-GGGGGATCCGGACTACAAGGACGACGATGACAAGATCCTAGCACAGATCCAATTGGTGCAGTCTGGACCTGAGCTG-3′

p5.2V_H-2

5′-GCCACCGCCAGAGCCACCTCCGCCTGAACCGCCTCCACCTGTCGACGATGAGGAGACTGTGAGAGTGGTGCCTTGGCCCCAGACGG-3′

p5.2V_L-1

5′-GGCGGTTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCGGATATCGACATTGTGATGTCACAGTCTCCATCCTCC-3′

p5.2V_L-2

5′-GGAGGAGGCCCCCTGGGCCCTGTTTTATTTCCAGCTTGGTCCCCCCTCCGAACGTGTACAGATTATAAGATTG-3′

pOKT3V_H-1

5′-GGGGGATCCGGACTACAAGGACGACGATGACAAGATCTCCCAGGTCCAGCTGCAGCAGTCTGGGGCTGAACTGGCAAGA-3′

pOKT3V_H-2

5′-GCCACCGCCAGAGCCACCTCCGCCTGAACCGCCTCCACCTGTCGACGATGAGGAGACTGTGAGAGTGGTGCCTTGGCCCCAGTAG-3′

pOKT3V_L-1

5′-GGCGGTTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCGGATATCCAAATTGTTCTCACCCAGTCTCCAGCAATC-3′

pOKT3V_L-2

5′-GGAGGAGGCCCCCTGGGCCCCGGTTTATTTCCAACTTTGTCCCCGAGCCGAACGTGAATGGGTTACTACTCC-3′

pOKT3V_H-GS

5′-GCCCAGGGGGCGGTGGAGGCGGTTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCGAAGATCTCCCAGGTCCAGCTGCAGCAGTCTGGG-3′

Sequences encoding (Gly₄Ser)₃ are underlined.

The restriction enzyme recognition sites are in italic.