Table 1. Addition of IVIGs to cultured B... | American Society of Hematology

Table 1.

Addition of IVIGs to cultured B lymphocytes did not affect cell viability

Time, days	Apoptotic cells and cell viability
	Control		IVIGs
	Annexin V/PI, %	Viable cells, %	Annexin V/PI, %	Viable cells, %
0	98.7	96	98.7	96
1	83.6	92	89.9	86
2	86.0	87	93.1	90
3	92.0	88	92.8	90
4	82.4	89	89.4	80

Time, days	Apoptotic cells and cell viability
	Control		IVIGs
	Annexin V/PI, %	Viable cells, %	Annexin V/PI, %	Viable cells, %
0	98.7	96	98.7	96
1	83.6	92	89.9	86
2	86.0	87	93.1	90
3	92.0	88	92.8	90
4	82.4	89	89.4	80

Purified human B lymphocytes were cultured with IL-4 and L4.5 cells in the presence of human serum albumin (9 mg/mL) and sucrose (15 mg/mL) or IVIGs (15 mg/mL). Apoptosis and necrosis levels were determined with annexin V and PI staining. Viable cell proportions were determined by viable cell count using trypan blue exclusion.

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