Primer sets used to amplify mtDNA
| Sets . | Position . | Length, bp . | Forward primer . | Reverse primer . |
|---|---|---|---|---|
| 1 | 15-921 | 907 | CACCCTATTAACCACTCACG | ACTTGGGTTAATCGTGTGACC |
| 2 | 873-1769 | 897 | GGTTGGTCAATTTCGTGCCAG | GCCAGGTTTCAATTTCTATCG |
| 3 | 1657-2660 | 1004 | CTTGACCGCTCTGAGCTAAAC | AGAGACAGCTGAACCCTCGTG |
| 4 | 2417-3557 | 1141 | CACTGTCAACCCAACACAGG | AGAAGAGCGATGGTGAGAGC |
| 5 | 3441-4728 | 1288 | ACTACAACCCTTCGCTGACG | TTATGGTTCATTGTCCGGAGAG |
| 6 | 4447-5882 | 1436 | TTGGTTATACCCTTCCCGTAC | GCTGAGTGAAGCATTGGACTG |
| 7 | 5700-6526 | 827 | TAAGCACCCTAATCAACTGGC | ATAGTGATGCCAGCAGCTAGG |
| 8 | 6426-7255 | 830 | GCCATAACCCAATACCAAACG | TTTCATGTGGTGTATGCATCG |
| 9 | 7215-8215 | 1001 | CGACGTTACTCGGACTACCC | GACGATGGGCATGAAACTG |
| 10 | 8164-9059 | 749 | CGGTCAATGCTCTGAAATCTGTG | GTGGCGCTTCCAATTAGGTG |
| 11 | 8903-9848 | 946 | CCCACTTCTTACCACAAGGC | GAAAGTTGAGCCAATAATGACG |
| 12 | 9754-10556 | 803 | AGTCTCCCTTCACCATTTCCG | GGAGGATATGAGGTGTGAGCG |
| 13 | 10386-11600 | 1215 | GGATTAGACTGAACCGAATTGG | CTGTTTGTCGTAGGCAGATGG |
| 14 | 11403-12189 | 787 | GACTCCCTAAAGCCCATGTCG | AAGCCTCTGTTGTCAGATTCAC |
| 15 | 11901-12876 | 976 | TGCTAGTAACCACGTTCTGGTG | GATATCGCCGATACGGTTG |
| 16 | 12793-13611 | 819 | TTGCTCATCAGTTGATGATACG | TCGAGTGCTATAGGCTTGTC |
| 17 | 13518-14388 | 871 | CATCATCGAAACCGCAAAC | TTAGCGATGGAGGTAGGATTCG |
| 18 | 14189-15396 | 1208 | ACAAACAATGGTCAACCAGTAAC | TTATCGGAATGGGAGGTGATTC |
| 19 | 15260-16451 | 1192 | AGTCCCACCCTCACACGATTC | GCGAGGAGAGTAGCACTCTTG |
| 20 | 16097-336 | 809 | TACATTACTGCCAGCCACCATG | TTAAGTGCTGTGGCCAGAAG |
| Sets . | Position . | Length, bp . | Forward primer . | Reverse primer . |
|---|---|---|---|---|
| 1 | 15-921 | 907 | CACCCTATTAACCACTCACG | ACTTGGGTTAATCGTGTGACC |
| 2 | 873-1769 | 897 | GGTTGGTCAATTTCGTGCCAG | GCCAGGTTTCAATTTCTATCG |
| 3 | 1657-2660 | 1004 | CTTGACCGCTCTGAGCTAAAC | AGAGACAGCTGAACCCTCGTG |
| 4 | 2417-3557 | 1141 | CACTGTCAACCCAACACAGG | AGAAGAGCGATGGTGAGAGC |
| 5 | 3441-4728 | 1288 | ACTACAACCCTTCGCTGACG | TTATGGTTCATTGTCCGGAGAG |
| 6 | 4447-5882 | 1436 | TTGGTTATACCCTTCCCGTAC | GCTGAGTGAAGCATTGGACTG |
| 7 | 5700-6526 | 827 | TAAGCACCCTAATCAACTGGC | ATAGTGATGCCAGCAGCTAGG |
| 8 | 6426-7255 | 830 | GCCATAACCCAATACCAAACG | TTTCATGTGGTGTATGCATCG |
| 9 | 7215-8215 | 1001 | CGACGTTACTCGGACTACCC | GACGATGGGCATGAAACTG |
| 10 | 8164-9059 | 749 | CGGTCAATGCTCTGAAATCTGTG | GTGGCGCTTCCAATTAGGTG |
| 11 | 8903-9848 | 946 | CCCACTTCTTACCACAAGGC | GAAAGTTGAGCCAATAATGACG |
| 12 | 9754-10556 | 803 | AGTCTCCCTTCACCATTTCCG | GGAGGATATGAGGTGTGAGCG |
| 13 | 10386-11600 | 1215 | GGATTAGACTGAACCGAATTGG | CTGTTTGTCGTAGGCAGATGG |
| 14 | 11403-12189 | 787 | GACTCCCTAAAGCCCATGTCG | AAGCCTCTGTTGTCAGATTCAC |
| 15 | 11901-12876 | 976 | TGCTAGTAACCACGTTCTGGTG | GATATCGCCGATACGGTTG |
| 16 | 12793-13611 | 819 | TTGCTCATCAGTTGATGATACG | TCGAGTGCTATAGGCTTGTC |
| 17 | 13518-14388 | 871 | CATCATCGAAACCGCAAAC | TTAGCGATGGAGGTAGGATTCG |
| 18 | 14189-15396 | 1208 | ACAAACAATGGTCAACCAGTAAC | TTATCGGAATGGGAGGTGATTC |
| 19 | 15260-16451 | 1192 | AGTCCCACCCTCACACGATTC | GCGAGGAGAGTAGCACTCTTG |
| 20 | 16097-336 | 809 | TACATTACTGCCAGCCACCATG | TTAAGTGCTGTGGCCAGAAG |