Table 2. Longitudinal analysis of... | American Society of Hematology

Table 2.

Longitudinal analysis of variants in an HTLV-1 immunodominant epitope, Tax 11-19, in 3 patients with HAM/TSP

Patient	Date	Proviral load^*	Frequency^†	Peptide sequence^‡	Peptide frequency	Percentage of variant epitope^2-153	Predicted HLA-A2 binding affinity^2-155
31	6/27/91	594	6.67	LLFGYPVYV	48/49	2.0	2406.2
				LLFRYPVYV	1/49		2406.2
	3/5/97	646	19.46	LLFGYPVYV	45/48	6.3	2406.2
				PLFGYPVYV	1/48		31.1
				LLYGYPVYV	1/48		2081.0
				LLFRYPVYV	1/48		2406.2
	3/10/99	506	11.00	LLFGYPVYV	48/49	2.0	2406.2
				LLFGHPVYV	1/49		2406.2
38	5/13/96	1631	2.43	LLFGYPVYV	48/51	5.9	2406.2
				LLLGYPVYV	1/51		2406.2
				LLFGYPIYV	2/51		2406.2
	2/21/97	2255	3.12	LLFGYPVYV	52/52	0.0	2406.2
	6/24/99	1810	2.20	LLFGYPVYV	39/49	20.4	2406.2
				LPFGYPVYV	1/49		15.7
				LLLGYPVYV	1/49		2406.2
				LLFRYPVYV	5/49		2406.2
				LLFGNPVYV	1/49		2406.2
				LLFGHPVYV	1/49		2406.2
				LLFGYPVHV	1/49		437.5
48	5/28/93	93	0.83	LLFGYPVYV	45/48	6.3	2406.2
				LLFRYPAYV	1/48		2406.2
				LLFGFPVYV	1/48		9143.4
				LLFGYPVCV	1/48		437.5
	10/21/96	606	5.47	LLFGYPVYV	48/51	5.9	2406.2
				LLFRYPVYV	1/51		2406.2
				LLFGYLVYV	1/51		5534.1
				LLFGYPVYE	1/51		0.5
	2/10/00	395	2.70	LLFGYPVYV	47/48	2.1	2406.2
				PLFGYPVYV	1/48		31.1

Patient	Date	Proviral load^*	Frequency^†	Peptide sequence^‡	Peptide frequency	Percentage of variant epitope^2-153	Predicted HLA-A2 binding affinity^2-155
31	6/27/91	594	6.67	LLFGYPVYV	48/49	2.0	2406.2
				LLFRYPVYV	1/49		2406.2
	3/5/97	646	19.46	LLFGYPVYV	45/48	6.3	2406.2
				PLFGYPVYV	1/48		31.1
				LLYGYPVYV	1/48		2081.0
				LLFRYPVYV	1/48		2406.2
	3/10/99	506	11.00	LLFGYPVYV	48/49	2.0	2406.2
				LLFGHPVYV	1/49		2406.2
38	5/13/96	1631	2.43	LLFGYPVYV	48/51	5.9	2406.2
				LLLGYPVYV	1/51		2406.2
				LLFGYPIYV	2/51		2406.2
	2/21/97	2255	3.12	LLFGYPVYV	52/52	0.0	2406.2
	6/24/99	1810	2.20	LLFGYPVYV	39/49	20.4	2406.2
				LPFGYPVYV	1/49		15.7
				LLLGYPVYV	1/49		2406.2
				LLFRYPVYV	5/49		2406.2
				LLFGNPVYV	1/49		2406.2
				LLFGHPVYV	1/49		2406.2
				LLFGYPVHV	1/49		437.5
48	5/28/93	93	0.83	LLFGYPVYV	45/48	6.3	2406.2
				LLFRYPAYV	1/48		2406.2
				LLFGFPVYV	1/48		9143.4
				LLFGYPVCV	1/48		437.5
	10/21/96	606	5.47	LLFGYPVYV	48/51	5.9	2406.2
				LLFRYPVYV	1/51		2406.2
				LLFGYLVYV	1/51		5534.1
				LLFGYPVYE	1/51		0.5
	2/10/00	395	2.70	LLFGYPVYV	47/48	2.1	2406.2
				PLFGYPVYV	1/48		31.1

Total numbers of variants of Tax 11-19 are 2, 1, 3, 9, 4, 1, 3, 2, and 1, in the order of amino acid position, respectively.

HTLV-1 proviral load is shown as copies/10⁴ PBMCs.

HTLV-1 Tax 11-19-specific T-cell frequency in CD8⁺ cell population is shown.

Amino acid sequence is presented by the single letter. Underlined amino acids indicate mutations.

Percentage of variant epitope in the sequenced clones is shown at each time point.

HLA-A2-binding affinity by dissociation half-time (minutes) of each peptide is predicted through the Internet (http://bimas.dcrt.nih.gov/moibio/hla_bind/).