Correlation between flanking DNA sequences mapped to human chromosomes and localization of FISH signals
| Clone . | FISH . | LM-PCR . | ||
|---|---|---|---|---|
| Vector sequence . | Human flanking sequence . | Database match* . | ||
| N2 | 12q14 | gtctttca | CTT CCC ATT CTG ACC | Not found |
| N3 | 1q41 | gtctttca | ATC AGA CCC TTC ATT | 1q41 |
| N4 | 20q11 | gtctttca | AAC CCA TTC ACC CTT | 20q11 |
| Clone . | FISH . | LM-PCR . | ||
|---|---|---|---|---|
| Vector sequence . | Human flanking sequence . | Database match* . | ||
| N2 | 12q14 | gtctttca | CTT CCC ATT CTG ACC | Not found |
| N3 | 1q41 | gtctttca | ATC AGA CCC TTC ATT | 1q41 |
| N4 | 20q11 | gtctttca | AAC CCA TTC ACC CTT | 20q11 |
Standard NCBI blast searches (http://www.ncbi.nlm.nih.gov/BLAST/) were done to identify cloned human DNA homologues to flanking sequences. About 92.8% of human genome sequences were finished and entered into databases at the time of preparation of this manuscript (EMBL genome monitoring table,http://www.ebi.ac.uk/genomes/mot/).