Cells were stained and analyzed by flow cytometry as described. The cell types were gated for their respective phenotypes and the percent of those cells expressing CD52 was determined. MFI of the CD52⁺ cells was also calculated by CellQuest software on the FACScan as a measure of CD52 epitope density on the cell surface. T cells were total CD4⁺ and CD8⁺lymphocytes. Monocytes were CD14⁺ PBMCs. All DC populations were large FSC, HLA-DR^bright cells. Immature DCs were CD83⁻, whereas mature DCs were CD83⁺ and additionally increased expression of CD40, CD80, and CD86. LCs were CD11b⁻, whereas moDCs and DDCs were CD11b⁺. The moDCs were differentiated from CD14⁺ monocytes as described, and CD34⁺ HPCs generated LCs and DDCs. DDCs nominally included both dermal (DDC) and interstitial (IDC) DCs. Fresh circulating DCs were defined as CD11c⁺, lineage-negative (CD3⁻, CD14⁻, CD16⁻, CD20⁻) PBMCs.