Tubular necrosis, vascular congestion, and neutrophil accumulation in the outer medulla in rats subjected to renal ischemia followed by reperfusion
| Treatment group . | Tubular necrosis, % . | Erythrocytes per HPF . | Neutrophil accumulation per HPF . |
|---|---|---|---|
| Sham-operated | 0.5 ± 0.8 | 77.7 ± 14.0 | 0.5 ± 0.8 |
| I/R + vehicle | 81.8 ± 6.0* | 332.7 ± 40.9* | 7.2 ± 3.9* |
| I/R + AT | 52.8 ± 11.1*,† | 169.7 ± 68.1*,† | 1.8 ± 1.3† |
| I/R + DEGR-F.Xa | 79.2 ± 5.0* | 299.0 ± 36.7* | 6.7 ± 2.3† |
| I/R + Trp49-modified AT | 78.3 ± 8.8* | 294.7 ± 38.8* | 6.2 ± 3.8* |
| I/R + IM | 86.5 ± 13.3* | 347.7 ± 74.0* | 8.2 ± 3.1* |
| I/R + IM + AT | 80.2 ± 11.0*,‡ | 304.4 ± 44.6*,‡ | 9.1 ± 3.0*,‡ |
| I/R + iloprost | 51.8 ± 16.4*,† | 158.5 ± 68.1*,† | 1.6 ± 1.0† |
| I/R + IM + iloprost | 54.5 ± 13.2*,†,1-153 | 166.3 ± 72.5*,†,1-153 | 1.9 ± 1.4†,1-153 |
| Treatment group . | Tubular necrosis, % . | Erythrocytes per HPF . | Neutrophil accumulation per HPF . |
|---|---|---|---|
| Sham-operated | 0.5 ± 0.8 | 77.7 ± 14.0 | 0.5 ± 0.8 |
| I/R + vehicle | 81.8 ± 6.0* | 332.7 ± 40.9* | 7.2 ± 3.9* |
| I/R + AT | 52.8 ± 11.1*,† | 169.7 ± 68.1*,† | 1.8 ± 1.3† |
| I/R + DEGR-F.Xa | 79.2 ± 5.0* | 299.0 ± 36.7* | 6.7 ± 2.3† |
| I/R + Trp49-modified AT | 78.3 ± 8.8* | 294.7 ± 38.8* | 6.2 ± 3.8* |
| I/R + IM | 86.5 ± 13.3* | 347.7 ± 74.0* | 8.2 ± 3.1* |
| I/R + IM + AT | 80.2 ± 11.0*,‡ | 304.4 ± 44.6*,‡ | 9.1 ± 3.0*,‡ |
| I/R + iloprost | 51.8 ± 16.4*,† | 158.5 ± 68.1*,† | 1.6 ± 1.0† |
| I/R + IM + iloprost | 54.5 ± 13.2*,†,1-153 | 166.3 ± 72.5*,†,1-153 | 1.9 ± 1.4†,1-153 |
Rats underwent 45 minutes of renal ischemia followed by reperfusion. Kidney samples were obtained 24 hours later. Data are expressed as mean ± SD values of 6 experiments.
HPF indicates high-power field; I/R, ischemia followed by reperfusion; AT, antithrombin; DEGR-F.Xa, dansyl glutamylglycylarginyl chloromethyl ketone-treated factor Xa; and IM, indomethacin.
P < .05 versus sham-operated group.
P < .05 versus vehicle-treated group.
P < .05 versus AT-treated group.
P < .05 versus IM-treated group.