Primer and probe combinations used for the real-time quantitative PCR
| AS | |
| Forward | 5′-GCC CAT GGT CTT GAA CT-3′ |
| Reverse | 5′-TTT GGT CGC CAG AGA AT-3′ |
| Probe | 5′-(FAM)-CTT GTC TCT GCC ACC AGA AAT GA-(TAMRA)-3′ |
| GAPDH | |
| Forward | 5′-GTC GGA GTC AAC GGA TT-3′ |
| Reverse | 5′-AAG CTT CCC GTT CTC AG-3′ |
| Probe | 5′-(FAM)-TCA ACT ACA TGG TTT ACA TGT TCC AA-(TAMRA)-3′ |
| AS | |
| Forward | 5′-GCC CAT GGT CTT GAA CT-3′ |
| Reverse | 5′-TTT GGT CGC CAG AGA AT-3′ |
| Probe | 5′-(FAM)-CTT GTC TCT GCC ACC AGA AAT GA-(TAMRA)-3′ |
| GAPDH | |
| Forward | 5′-GTC GGA GTC AAC GGA TT-3′ |
| Reverse | 5′-AAG CTT CCC GTT CTC AG-3′ |
| Probe | 5′-(FAM)-TCA ACT ACA TGG TTT ACA TGT TCC AA-(TAMRA)-3′ |