Characteristics of the transplanted apoE−/−animals
| P-selectin genotype . | Plts . | ECs . | Cholesterol level, mg/dL . | WBC count, × 103 cells/μL . | Plt count, × 103 cells/μL . | Spleen weight, g . |
|---|---|---|---|---|---|---|
| wt-wt | wt | wt | 507.3 ± 38.8 | 9.6 ± 1.7 | 1389 ± 81 | 0.087 ± 0.012 |
| ko-wt | ko | wt | 512.8 ± 65 | 8.5 ± 1.2 | 1216 ± 112 | 0.138 ± 0.006 |
| wt-ko | wt | ko | 411.1 ± 17 | 5.4 ± 1.9 | 1159 ± 77 | 0.094 ± 0.013 |
| ko-ko | ko | ko | 474.2 ± 48.1 | 6.0 ± 1.1 | 1152 ± 143 | 0.178 ± 0.022 |
| P-selectin genotype . | Plts . | ECs . | Cholesterol level, mg/dL . | WBC count, × 103 cells/μL . | Plt count, × 103 cells/μL . | Spleen weight, g . |
|---|---|---|---|---|---|---|
| wt-wt | wt | wt | 507.3 ± 38.8 | 9.6 ± 1.7 | 1389 ± 81 | 0.087 ± 0.012 |
| ko-wt | ko | wt | 512.8 ± 65 | 8.5 ± 1.2 | 1216 ± 112 | 0.138 ± 0.006 |
| wt-ko | wt | ko | 411.1 ± 17 | 5.4 ± 1.9 | 1159 ± 77 | 0.094 ± 0.013 |
| ko-ko | ko | ko | 474.2 ± 48.1 | 6.0 ± 1.1 | 1152 ± 143 | 0.178 ± 0.022 |
There were no significant differences due to the P-selectin genotype in serum total cholesterol, white blood cell (WBC) or platelet counts among the 4 groups of transplanted animals.
In contrast, the genotype of the chimeric animals affected splenic weights. Platelet P-selectin appeared to be the most important factor determining the weight of the organ (P determined by ANOVA and Fisher PLSD post-hoc test: wt-wt, ko-ko < .0001; wt-wt, ko-wt < .02; wt-ko, ko-wt < .04; wt-wt, wt-ko = .74; ko-wt, ko-ko < .06). Thus, the presence of platelet P-selectin appears to prevent the appearance of the enlarged spleen seen in apoE−/−P-sel−/− mice.8
Plts indicates platelets; ECs, endothelial cells; WBC, white blood cell; n = 6-10.