Table 1. SMCs regulate ICAM-1, VCAM-1,... | American Society of Hematology

Table 1.

SMCs regulate ICAM-1, VCAM-1, and E-selectin gene expression in cocultured ECs via close interaction between these cocultured cells

Adhesion molecule	Control			Shear
Adhesion molecule	EC/SMC	EC/slide/SMC	EC/φ + med	EC/φ	EC/SMC	EC/slide/SMC
ICAM-1	561 ± 86^*	98 ± 16^†	102 ± 16^†	339 ± 40^*	88 ± 17^†	296 ± 32^*
VCAM-1	1153 ± 264^*	103 ± 19^†	95 ± 8^†	92 ± 11	42 ± 11^*,^†	95 ± 14
E-selectin	967 ± 213^*	108 ± 12^†	97 ± 13^†	124 ± 31	93 ± 16^†	109 ± 18

Adhesion molecule	Control			Shear
Adhesion molecule	EC/SMC	EC/slide/SMC	EC/φ + med	EC/φ	EC/SMC	EC/slide/SMC
ICAM-1	561 ± 86^*	98 ± 16^†	102 ± 16^†	339 ± 40^*	88 ± 17^†	296 ± 32^*
VCAM-1	1153 ± 264^*	103 ± 19^†	95 ± 8^†	92 ± 11	42 ± 11^*,^†	95 ± 14
E-selectin	967 ± 213^*	108 ± 12^†	97 ± 13^†	124 ± 31	93 ± 16^†	109 ± 18

ECs cultured alone (EC/φ) or cocultured with SMCs grown on a cover slip (EC/slide/SMC) or without a cover slip (EC/SMC) were maintained in static condition or exposed to shear stress of 12 dyne/cm² for 6 hours and their mRNA expression was determined by RT-PCR analysis, as described in “Materials and methods.” Amplification of the cDNA was performed in parallel samples using human GAPDH primers. ECs that were incubated in a static condition with culture medium collected from the EC/SMC coculture (EC/φ + med) did not significantly increase their expression of ICAM-1, VCAM-1, or E-selectin mRNA. Data on band density normalized to GAPDH RNA levels are presented as percentages of the results for the control EC/φ (taken as 100%). The results shown are mean ± SEM from 4 independent experiments in each group.

P < .05 vs control EC/φ.

†

P < .05 vs control EC/SMC.

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