Summary of hepcidin derivative bioactivity
. | Flow cytometry . | . | Ferritin ELISA . | . | ||
|---|---|---|---|---|---|---|
| Peptide . | Hepcidin-25 activity, % . | P . | Hepcidin-25 activity, % . | P . | ||
| Hep24 | 95 ± 4 | .018 | 77 ± 19 | .034 | ||
| Hep23 | 88 ± 4 | < .001 | 47 ± 28 | .003 | ||
| Hep22 | 46 ± 6 | < .001 | 13 ± 16 | < .001 | ||
| Hep21 | 22 ± 3 | < .001 | 3 ± 6 | < .001 | ||
| Hep20 | 12 ± 8 | < .001 | 3 ± 6 | < .001 | ||
| Hep3 | –6 ± 10 | < .001 | –2 ± 10 | < .001 | ||
| Hep6 | 1 ± 3 | < .001 | –5 ± 5 | < .001 | ||
| Hep26 | 100 ± 1 | NS | – | – | ||
| delKT | 90 ± 7 | .01 | 87 ± 18 | NS | ||
| C7A/C23A | 85 ± 3 | < .001 | 107 ± 12 | NS | ||
| C11A/C19A | 92 ± 6 | .014 | 101 ± 9 | NS | ||
| C13A/C14A | 92 ± 12 | NS | 84 ± 21 | NS | ||
| 1SS | 54 ± 5 | < .001 | 36 ± 29 | < .001 | ||
| G71D | 108 ± 1 | < .001 | 91 ± 11 | NS | ||
| K83R | 101 ± 3 | NS | 107 ± 17 | NS | ||
| zebrafish | 106 ± 10 | NS | 103 ± 8 | NS | ||
| hep25 + 20; 5 μM | 102 ± 2 | .05 | – | – | ||
| hep3; 20 μM | –10 ± 7 | < .001 | – | – | ||
| hep25 + 3; 20 μM | 102 ± 3 | NS | – | – | ||
| hep6; 20 μM | 6 ± 4 | < .001 | – | – | ||
| hep25 + 6; 20 μM | 96 ± 4 | .047 | – | – | ||
. | Flow cytometry . | . | Ferritin ELISA . | . | ||
|---|---|---|---|---|---|---|
| Peptide . | Hepcidin-25 activity, % . | P . | Hepcidin-25 activity, % . | P . | ||
| Hep24 | 95 ± 4 | .018 | 77 ± 19 | .034 | ||
| Hep23 | 88 ± 4 | < .001 | 47 ± 28 | .003 | ||
| Hep22 | 46 ± 6 | < .001 | 13 ± 16 | < .001 | ||
| Hep21 | 22 ± 3 | < .001 | 3 ± 6 | < .001 | ||
| Hep20 | 12 ± 8 | < .001 | 3 ± 6 | < .001 | ||
| Hep3 | –6 ± 10 | < .001 | –2 ± 10 | < .001 | ||
| Hep6 | 1 ± 3 | < .001 | –5 ± 5 | < .001 | ||
| Hep26 | 100 ± 1 | NS | – | – | ||
| delKT | 90 ± 7 | .01 | 87 ± 18 | NS | ||
| C7A/C23A | 85 ± 3 | < .001 | 107 ± 12 | NS | ||
| C11A/C19A | 92 ± 6 | .014 | 101 ± 9 | NS | ||
| C13A/C14A | 92 ± 12 | NS | 84 ± 21 | NS | ||
| 1SS | 54 ± 5 | < .001 | 36 ± 29 | < .001 | ||
| G71D | 108 ± 1 | < .001 | 91 ± 11 | NS | ||
| K83R | 101 ± 3 | NS | 107 ± 17 | NS | ||
| zebrafish | 106 ± 10 | NS | 103 ± 8 | NS | ||
| hep25 + 20; 5 μM | 102 ± 2 | .05 | – | – | ||
| hep3; 20 μM | –10 ± 7 | < .001 | – | – | ||
| hep25 + 3; 20 μM | 102 ± 3 | NS | – | – | ||
| hep6; 20 μM | 6 ± 4 | < .001 | – | – | ||
| hep25 + 6; 20 μM | 96 ± 4 | .047 | – | – | ||
The results, expressed as percentage of the hep25 activity, are the mean ± SD of 3 to 6 independent experiments. The values obtained by flow cytometry represent the ability of peptide to cause degradation of ferroportin-GFP, and the values obtained by ferritin ELISA represent the peptides' ability to cause cellular iron retention. Each peptide was added at the concentration of 1 μM, unless indicated otherwise. Paired t test was used for statistical analysis for each peptide in comparison to hep25.
NS indicates not significant (> .05); –, not done.