Effects of PDE4 LMWI on relative percent of CD3/CD28-induced proliferation and IL-2 production of CD3+ wild-type and PKCθ- or PKCα-deficient T cells
. | Proliferation . | . | IL-2 secretion . | . | ||
|---|---|---|---|---|---|---|
. | DMSO control . | 5 nM PDE4 LMWI . | DMSO control . | 5 nM PDE4 LMWI . | ||
| wt+/+ | 100 | 35 ± 17 | 100 | 97 ± 73 | ||
| PKCα-/- | 100 | 38 ± 10 | 100 | 89 ± 48 | ||
| PKCθ-/- | 100 | 36 ± 15 | 100 | 33 ± 38 | ||
. | Proliferation . | . | IL-2 secretion . | . | ||
|---|---|---|---|---|---|---|
. | DMSO control . | 5 nM PDE4 LMWI . | DMSO control . | 5 nM PDE4 LMWI . | ||
| wt+/+ | 100 | 35 ± 17 | 100 | 97 ± 73 | ||
| PKCα-/- | 100 | 38 ± 10 | 100 | 89 ± 48 | ||
| PKCθ-/- | 100 | 36 ± 15 | 100 | 33 ± 38 | ||
Effects of PDE4 LMWI (at 5 nM) on relative percent of CD3/CD28-induced proliferation and IL-2 production of CD3+ wild-type and PKCθ- or PKCα-deficient T cells (DMSO control was set as 100% in each genotype for direct comparison purposes). Basically similar results have been obtained with PDE4 LMWI at 0.4 nM (not shown). Statistical analysis of 3 experiments is shown.